Browsing by Author "Kongprakhon, Phinyarat"

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    Validation of rice blast resistance genes in barley using a QTL mapping population and near-isolines
    (Breeding Science, 2009) Steffenson, Brian; Kongprakhon, Phinyarat; Cuesta-Marcos, Alfonso; Hayes, Patrick M.; Richardson, Kelley L.; Sirithunya, Pattama; Sato, Kazuhiro; Toojinda, Theerayuth
    There are prior reports of Pyricularia grisea—the causal agent of blast of rice—causing disease in barley. In order to determine the specificity of this resistance in barley, we extended our previous mapping efforts to include blast isolates from barley and rice grown in Thailand and we assessed two resistance phenotypes: leaf blast (LB) and neck blast (NB). The largest-effect resistance QTL, on chromosome 1H, was associated with NB and LB and is located in a region rich in resistance genes, including QTL conferring resistance to stripe rust (incited by Puccinia striiformis f. sp. hordei) and the mildew (Blumeria graminis f. sp. hordei) resistance gene Mla. The LB, NB and mildew resistance alleles trace to one parent (Baronesse) whereas the stripe rust resistance allele traces to the other parent (BCD47) of the mapping population. Baronesse is the susceptible recurrent parent of a set of near-isogenic lines (NILs) for three stripe rust resistance QTL, including one on 1H. Unigene (EST) derived single nucleotide polymorphism haplotypes of these NILs were aligned with the blast mapping population QTL using Mla as an anchor. Baronesse and all NILs without the 1H introgression were resistant to LB and NB. However, two NILs with the 1H introgression were resistant to LB and NB. Both are resistant to stripe rust. Therefore, the QTL conferring resistance to stripe rust is separable by recombination from the blast resistance QTL.