Browsing by Author "Jin, Yue"

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    Aecial and telial host specificity of Puccinia coronata var. coronata, a Eurasian crown rust fungus of two highly invasive wetland species in North America
    (Plant Disease, 2023-08) Greatens, Nicholas; Jin, Yue; Pablo, D. Olivera
    The Eurasian crown rust fungus Puccinia coronata var. coronata (Pcc) was recently reported in North America and is widespread across the Midwest and Northeast United States. Pcc is a close relative of major pathogens of oats, barley, and turfgrasses. It infects two highly invasive wetland plants, glossy buckthorn (Frangula alnus) and reed canarygrass (Phalaris arundinacea) and could be useful as an augmentative biological control agent. We conducted large greenhouse trials to assess the host specificity of Pcc and determine any threat to cultivated cereals, turfgrasses, or native North American species. A total of 1,830 accessions of cereal crop species and 783 accessions of 110 other gramineous species were evaluated. Young plants were first inoculated with a composite uredinial inoculum derived from aecia. Accessions showing sporulation were further tested with pure urediniospore isolates. Sixteen potential aecial hosts in the families Rhamnaceae and Elaeagnaceae were tested for susceptibility through inoculation with germinating teliospores. Thirteen grass species within five genera in the tribe Poeae, Apera, Calamagrostis, Lamarckia, Phalaris and Puccinellia, and four species in Rhamnaceae, Frangula alnus, F. californica, F. caroliniana and Rhamnus lanceolata, were found to be susceptible to Pcc, with some species native to North America. All assessed crop species and turfgrasses were resistant. Limited sporulation, however, was observed on some resistant species within Poeae and four other tribes, Brachypodieae, Bromeae, Meliceae, and Triticeae. Among these species are oats, barley, and Brachypodium distachyon, suggesting the possible use of Pcc in studies of non-host resistance.
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    Linkage between the Rpg1 gene for stem rust resistance and the f5 locus on barley chromosome
    (Crop Science, 1993) Steffenson, Brian; Jin, Yue; Franckowiak, Jerome D
    Linkage studies can expedite the transfer of agronomically important genes in breeding programs. A study was conducted to determine the linkage relationship between loci segregating for stem rust (Puccinia graminis Pers.:Pers. f. sp. tritici Eriks. & E. Henn.) resistance (Rpg1) and a chlorina mutant (f5), and to confirm the linkage among Rpg1, br1 (brachytic) and fc (chlorina seedling). ‘Bowman’ barley (Hordeum vulgare L.) was crossed to genetic stocks possessing br1, fc, and f5, respectively. Estimates of linkage distances were 9.6 ± 1.4% between Rpg1 and br1, 13.6 ± 1.8% between Rpg1 and fc, and 25.9 ± 2.6% between Rpg1 and f5. The linkage between Rpg1 and f5 was established.
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    Puccinia coronata var. coronata , a Crown Rust Pathogen of Two Highly Invasive Species, Is Detected Across the Midwest and Northeastern United States
    (Plant Disease, 2023-07) Greatens, Nicholas; Klejeski, Nick; Szabo, Les J; Jin, Yue; Olivera, Pablo D
    Puccinia coronata var. coronata (Pcc) causes crown rust disease of glossy buckthorn (Frangula alnus) and reed canarygrass (Phalaris arundinacea), two highly invasive plant species in North America. Pcc is closely related to major pathogens of cereals, turfgrasses, and forage grasses. It occurs throughout Europe but was first recorded in North America in 2013. Where its hosts co-occur, such as in wetlands in the Twin Cities metro area in Minnesota, we have observed Pcc causing significant infection that results in defoliation and fruit loss in glossy buckthorns and premature leaf senescence in reed canarygrass. In this research, we mapped the distribution of this likely recently introduced rust fungus and provided a description of disease signs and symptoms and pathogen morphology. Samples were acquired by two primary means: by surveys in Minnesota and by correspondence with users of iNaturalist.org, a social network for nature enthusiasts and community scientists. With an Oxford Nanopore MinION, we sequenced two to four loci from 22 samples across 13 states and identified samples by phylogenetic analysis and sequence similarity. Notably, four pure isolates appear to have intragenomic variation of the ITS region. We found that Pcc is present throughout the range of glossy buckthorn in the eastern United States. In Minnesota, Pcc is not common outside the range of glossy buckthorn despite the presence of susceptible grass hosts.
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    SNP data of 18SPA092-1 and 25 reference isolates of the wheat stem rust fungus, Puccinia graminis f. sp. tritici
    (2022-02-04) Olivera Firpo, Pablo D; Villegas, Dolors; Cantero-Martinez, Carlos; Szabo, Les J; Rouse, Matthew N; Luster, Douglas; Bartaula, Radhika; Lopes, Marta; Jin, Yue; oliv0132@umn.edu; Olivera Firpo, Pablo D; Wheat stem rust research project / USDA-ARS Cereal Disease Laboratory and University of Minnesota
    This dataset includes 1838 SNP loci of 26 isolates of the wheat stem rust fungus, Puccina graminis f .sp. tritici. This data was used to assess the genetic relationship of a unique fungal isolate (18SPA092-1, race TKHBK) derived from a wheat stem rust sample collected in Spain in 2018 with a set of 25 reference isolates from previously defined genetic clades. Results indicate that isolate 18SPA092-1 had a unique multilocus genotype and constitutes a previously unknown lineage. Data is being released now in conjunction with publication of a primary research paper that describes this work.
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    SNP data of 56 Kazakhstani and 23 reference isolates of the wheat stem rust fungus, Puccinia graminis f. sp. tritici
    (2022-03-09) Olivera Firpo, Pablo; Szabo, Les J; Kokhmetova, Alma; Morgunov, Alexey; Luster, Douglas; Jin, Yue; oliv0132@umn.edu; Olivera Firpo, Pablo, D; Wheat stem rust research project | USDA ARS Cereal Disease Lab and University of Minnesota
    This dataset includes 2310 SNP loci of 79 isolates of the wheat stem rust fungus, Puccina graminis f .sp. tritici (Pgt). This data was used to assess the genetic diversity of a Pgt population (56 isolates) from Kazakhstan derived from stem rust samples collected in 2015-2017 wheat growing seasons. Twenty-three reference isolates from previously defined genetic clades were included in the analysis. Results indicate that the Pgt population form Kazakhstan is highly diverse and most of the isolates are of sexual origin. Data is being released now in conjunction with publication of a primary research paper that describes this work.
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    Sources of resistance to pathotype QCC of Puccinia graminis f. sp. tritici in barley
    (Crop Science, 1994) Steffenson, Brian; Jin, Yue; Fetch, Thomas G.
    The occurrence of a wheat stem rust (Puccinia graminis Pers.:Pers. f. sp. tritici Eriks. & E. Henn.) pathotype (Pgt-QCC) with virulence for the Rpg1 gene in barley (Hordeum vulgare L.) necessitated the search for resistant barley germplasm. From preliminary screenings of over 18 000 barley accessions, 13 lines were identified as possessing resistance to pathotype QCC: ‘Diamond’, ‘Hietpas 5’, Q21861, PC 11, PC 84, PC 249, PC 250, CI 5541, PI 452406, PI 452421, PI 477843, PI 477854, and PI 477860. This study was conducted to further characterize the reaction of the selected lines to pathotype QCC. The reaction was assessed by evaluating infection types at the seedling stage and infection responses at the adult plant stage in the greenhouse, and by evaluating disease severity and infection responses at the adult plant stage in the field compared to susceptible cultivars. Most lines exhibited low to intermediate infection types at the seedlings stage and moderately resistant to moderately susceptible infection responses at the adult plant stage in the greenhouse experiments. Among the selected lines, Q21861 exhibited the highest level of resistance at both the seedling and adult plant stages. These lines may provide an adequate level of resistance to pathotype QCC for cultivar development.