Between Dec 19, 2024 and Jan 2, 2025, datasets can be submitted to DRUM but will not be processed until after the break. Staff will not be available to answer email during this period, and will not be able to provide DOIs until after Jan 2. If you are in need of a DOI during this period, consider Dryad or OpenICPSR. Submission responses to the UDC may also be delayed during this time.

Browsing by Author "Heiberger, Laura"

Now showing 1 - 1 of 1
  • Thumbnail Image
    Item
    In vitro direct reprogramming of mouse biliary epithelial cells towards an insulin-producing state
    (2015-06) Heiberger, Laura
    Type I diabetes is an autoimmune disease resulting in the destruction of beta cells, the cell type in the body that produces insulin in the pancreas. Direct reprogramming can enable respecification of non-beta cells to produce insulin. The reprogramming vector, Ad-PNM, that expresses the genes Pdx1, Ngn3, and Mafa has reprogrammed non-beta cells of the pancreas and liver to produce insulin and restore normoglycemia in diabetic mice in vivo. Another possible approach is in vitro reprogramming with the same vector using cultured liver cells. Specifically, in this project biliary epithelial cells were isolated from mice, examined for Sox9 expression and treated with Ad-PNM to induce reprogramming.