Browsing by Author "He, Huan"

Now showing 1 - 2 of 2
  • Thumbnail Image
    Item
    Determination of the antibiotic and antibiotic resistance footprint in surface water environment of a metropolitan area: Effects of anthropogenic activities
    (2022-09-26) He, Huan; Bueno, Irene; Kim, Taegyu; Wammer, Kristine H.; LaPara, Timothy M.; Singer, Randall S.; Beaudoin, Amanda; Arnold, William A.; heh@umn.edu; He, Huan; University of Minnesota Department of Civil, Environmental, and Geo- Engineering; University of Minnesota Department of Veterinary and Biomedical Sciences; University of St Thomas Department of Chemistry; Minnesota Department of Health
    This study investigated geospatial distributions of antibiotics and antibiotic resistance genes (ARGs) in surface waters and their associations with anthropogenic activities. During July‒October 2020, the concentrations of antibiotics (water and sediment) and ARGs (sediment) were measured at 39 sites in the Twin Cities metropolitan area (Minnesota) that experience a gradient of impacts related to human activities. For water samples, the number of antibiotics detected and the concentrations of certain antibiotics (e.g., sulfonamides) positively correlated with urbanization indicators (e.g., urban percentage, population density, number of wastewater discharge points; ρ =0.32‒0.46, p =0.003‒0.04) and negatively correlated with undeveloped land indicators (e.g., forest; ρ =-0.34‒-0.62, p =<0.00001‒0.04). Antibiotics in sediments exhibited geospatial distribution different from that in corresponding water samples and exhibited no associations with anthropogenic factors. Relative abundances of ARGs were not associated with anthropogenic factors, but several ARGs (e.g., blaoxa, mexB, and sul2) were inversely related to the organic content of sediments (ρ =-0.38‒-0.44, p =0.01‒0.04). Strong correlations were found among relative abundances of various ARGs and intI1 (ρ ≥ 0.67, p < 0.05), highlighting their co-occurrence in (sub)urban surface waters. These results identified promising anthropogenic/environmental factors for predicting antibiotic geospatial distributions and useful gene markers to monitor ARGs in surface waters.
  • Thumbnail Image
    Item
    Persistence of Antibiotics in the Natural Environment: Scoping Review Protocol
    (2021) Bueno, Irene; He, Huan; Nault, Andre J.; Ziemann, Sarah; Degn, Lauren; Arnold, William A.; Wammer, Kristine H.
    Background: Antibiotics and their metabolites released into aquatic and soil environments have the potential to affect their microbial communities and can be a selection pressure to drive antimicrobial resistance emergence and spread. However, data about the persistence of antibiotics and metabolites into the natural environment are still lacking. Objectives: The goal of this manuscript is to describe the protocol that will be used to conduct a formal scoping review of the current literature to address the following question: “What is known from the existing literature about degradation of a selected group of antibiotic compounds in water, sediment, and soil?”. Eligibility criteria: Eligible studies will be primary research, in English, from any geographic location, published between 2000-2020, include water, sediment, and/or soil samples, were conducted in natural systems and/or laboratory studies with relevant data applied to natural systems, include data related to transformation by sunlight, biodegradation, and/or sorption processes, and include data for any of the following compounds: i) sulfonamides: sulfachlorpyridazine, sulfadiazine, sulfadimethoxine, sulfamethazine, sulfamethoxazole, sulfapyridine; ii) macrolides: erythromycin, roxithromycin, tylosin, azithromycin; iii) tetracyclines: chlortetracycline, doxycycline, oxytetracycline, tetracycline; iv) fluoroquinlones: ciprofloxacin, enrofloxacin, norfloxacin, ofloxacin; v) beta-lactams: penicillins; vi) others: carbadox, trimethoprim, lincomycin.