Izri, ZianeNoireaux, Vincent2024-11-202024-11-202024-11-20https://hdl.handle.net/11299/267840Data includes .tif and .xlsx files. These data will allow any manuscript reader full access to the experimental results that lead to the statements and conclusions made in the manuscript. Should the reader try to reproduce the experiments presented in the manuscript, they will have a reference to compare their experimental results.The data shared in this repository consists mainly of the collection of the original fluorescence microscopy pictures of the PSTXTL system presented in the manuscript together with the product of their processing and analysis. Those pictures were acquired through three different channels: Bright Field, FITC and Texas Red. The bright field images inform on the presence of phase-separated droplets, while the FITC and Texas Red channels inform on the content of the phase-separated droplets, depending on the fluorophores used. The results of the fluorometry analysis are also present in the data collection.CC0 1.0 Universalhttp://creativecommons.org/publicdomain/zero/1.0/cell-free transcription-translationliquid-liquid phase separationsynthetic cellprotocellsynthetic organellesbiocompatible polymersDatasethttps://doi.org/10.13020/9gbe-2r60