Kaarthik, Saravanan Sujit2024-04-302024-04-302024https://hdl.handle.net/11299/262854University of Minnesota M.S. thesis. 2024---. Major: Mechanical Engineering. Advisor: Michael McAlpine. 1 computer file (PDF); v, 71 pages.The ability to manufacture large-scale integrated microfluidic devices (mLSI) in an automated fashion with high throughput could impact numerous areas, including single-cell assays, drug discovery, and multi-sample analysis of human fluids. Conventional microfluidics fabrication is labor-intensive and requires the use of specialized facilities. Our group previously pioneered a method to 3D print microfluidic channels and valves by extruding silicone filaments in angular stacks. This technique faced limitations in scaling due to slow printing speed (1 mm/s) and inability to generate multiplexed flows. Here, we present an approach to 3D print mLSI devices that introduces an innovative method to reinforce channels locally and reduce the printing time 20-fold by doubling the extrusion diameter of the filaments. This allows for the incorporation of a Boolean design strategy that requires specific valves to remain open when actuated. This work paves the way for point-of-need mLSI production for medical diagnostics and disease detection.en3D printingmicrofluidicsmLSIpneumatic valveThesis or Dissertation