Dorr, Casey2024-01-052024-01-052023-08https://hdl.handle.net/11299/259530University of Minnesota M.S. thesis. August 2023. Major: Biomedical Informatics and Computational Biology. Advisor: Ajay Israni. 1 computer file (PDF); viii, 93 pages.This master’s thesis focuses on the work completed during my K01 award Genetic Variants Associated with Tacrolimus (TAC) metabolism in Kidney Transplant Recipients. In Chapter 1, I discuss the significance and innovations of this project. In Chapter 2, I present reformatted manuscript published in the Pharmacogenomics Journal titled: Identification of genetic variants associated with tacrolimus metabolism in kidney transplant recipients by extreme phenotype sampling and next generation sequencing. In Chapter 3, I present a reformatted manuscript published in Drug Metabolism and Disposition titled: CRISPR/Cas9 genetic modification of CYP3A5 *3 in human hepatocytes leads to cell lines with increased midazolam and tacrolimus metabolism. Chapter 4 is the general conclusions, future directions and take away messages.engeneticskidneymodelingsequencingtacrolimustransplantThesis or Dissertation