Chu, Hon Man Hamlet2010-02-222010-02-222009-11https://hdl.handle.net/11299/58288University of Minnesota Ph.D. dissertation December 2009. Major: Microbiology, Immunology and Cancer Biology. Advisor: Marc K Jenkins, Ph.D., 1 computer file (PDF); ix, 104 pages.Cell-mediated immune responses are initiated when a population of pre-immune (or naïve) T cells recognizes their cognate ligands in the form of a specific peptide bound to a self-major histocompatibility complex molecule (pMHC). This recognition is made possible by highly specific Tcell receptors (TCR) on individual T cell clones specific for a given pMHC complex. The pre-immune T cell repertoire is comprised of populations specific for at least 100,000 different pMHC, each containing multiple clones. It is important to understand the composition of this repertoire because it is the repository of the host’s potential for future cell-mediated immune responses to microbes and tumors, and in some cases its own tissues. However, the study of individual pre-immune pMHC-specific T cell populations within such a diverse repertoire has been extremely difficult because of their small size. A novel soluble pMHC-based magnetic enrichment technique was therefore developed to analyze naive T cell populations in mice and humans. Using this procedure, different pMHCII-specific naive CD4+ T cell populations were identified and enumerated. The size of these populations was found to vary depending on pMHC specificity. Additionally, these differences were directly proportional to the magnitude of the primary CD4+ T cell response after immunization with the relevant peptide.Thus, variation in naive T cell frequencies can explain why some peptides give rise to greater T cell responses than others. We explored this issue by enumerating pMHCII-specific CD4+ T cell populations that normally number 20 or 200 cells per mouse. Thymic positive or negative selection was required for optimizing the absolute size of each population but did not alter the 10-fold ratio between the two populations. Large naïve population size was related to the presence of certain amino acids at TCR contact sites within the peptide. These results suggest that certain MHCII-bound peptides are immunodominant because they contain amino acids with chemical properties that foster binding to many TCRs.en-USMajor Histocompatibility ComplexT cell receptorT cell repertoireMicrobiology, Immunology and Cancer Biology.Thesis or Dissertation