Wittman, Jacob TAukema, Brian HDuan, Jian JVenette, Robert C2021-04-262021-04-262021-04-26https://hdl.handle.net/11299/219452This repository contains data and code required to reproduce the analyses and figures in the associated manuscript.From publication: Evaluating the cold tolerance of biological control agents is often necessary to optimize their release and performance. We used field and laboratory assays to determine the cold hardiness of the parasitoid Spathius galinae Belokobylskij & Strazanac, an approved classical biological control agent of emerald ash borer (Agrilus planipennis Fairmaire) in North America. Supercooling points and lower lethal temperature of mature (cocooned) S. galinae larvae were measured in controlled cooling assays in the laboratory. Most S. galinae larvae died after reaching their supercooling point, which occurred at -25.0°C on average. Several larvae, however, initiated freezing but later eclosed, suggesting S. galinae may be partially freeze tolerant. Supercooling points were not affected by chilling rate. In the winter of 2019 – 2020, we monitored development of mature S. galinae larvae in ash segments above and beneath the snow in three locations in Minnesota, USA. Nearly 100% of S. galinae larvae died after air temperatures reached -29°C in Minnesota. Using models developed from our data, we forecast eclosion rates of S. galinae based on minimum winter temperatures across the range of ash (Fraxinus spp.) in North America. Our results indicate that S. galinae populations may suffer high overwintering mortality in areas where winter temperatures regularly decrease below -28°C, but a small portion of the population may be able to survive lower temperatures.Attribution-ShareAlike 3.0 United Stateshttp://creativecommons.org/licenses/by-sa/3.0/us/cold toleranceemerald ash borerparasitoidsupercoolingeclosionDatasethttps://doi.org/10.13020/rhzw-dk37