Holten, Matthew PFonseca, Dallas RCosta, Kyle C2021-02-052021-02-052021-02-05https://hdl.handle.net/11299/218309These data are the raw mass spectrometry data files for independent genotypes. The corresponding genotypes to sample name are DF1=S2Δupt, DF2=JJΔupt top band, DF3= JJΔupt bottom band, DF4=JJΔuptΔaglB. Images may be opened using open source software ImageJ.Most microbial organisms grow as surface-attached communities known as biofilms. However, the mechanisms whereby methanogenic archaea grow attached to surfaces have remained understudied. Here, we show that the oligosaccharyltransferase AglB is essential for growth of Methanococcus maripaludis strain JJ on glass or metal surfaces. AglB glycosylates several cellular structures such as pili, archaella, and the cell surface layer (S-layer). We show that the S-layer of strain JJ, but not strain S2, is a glycoprotein, that only strain JJ was capable of growth on surfaces, and that deletion of aglB blocked S-layer glycosylation and abolished surface-associated growth. A strain JJ mutant lacking structural components of the type IV-like pilus did not have a growth defect under any conditions tested, while a mutant lacking the pre-flagellin peptidase (∆flaK) was only defective for surface growth when formate was provided as the sole electron donor. Finally, for strains that are capable of Fe0 oxidation, we show that deletion of aglB decreases the rate of anaerobic Fe0 oxidation, presumably due to decreased association of biomass with the Fe0 surface. Together, these data provide an initial characterization of surface-associated growth in a member of the methanogenic archaea.CC0 1.0 UniversalMethanococcus maripaludisSurface Layer ProteinSurface Associated GrowthDatasethttps://doi.org/10.13020/fhxh-n267