Wei, GuanjuYang, Judy2023-05-042023-05-042023-05-04https://hdl.handle.net/11299/253957The raw images folder contains the raw images collected using Nikon confocal microscope. The Matlab codes are used for processing these raw images to calculate the biofilm thickness and biofilm area coverage. The data folder contains the data after processing.This dataset consists of the Matlab codes, experimental data, and raw images of the biofilm development experiments under fluctuating flow conditions. They are all collected in the Saint Anthony Falls Laboratory at the University of Minnesota. The codes are used for processing the raw images to calculate the biofilm thickness and biofilm area coverage. The experimental data file contains the data after processing. The image file contains the raw images collected during the experiments using the Nikon confocal microscope.CC0 1.0 UniversalBiofilmMicrofluidicsFluctuating flowDatasethttps://doi.org/10.13020/jwgn-4078