Soltau, Alexander D.Malisetti, NithyaWinslow, Peter JSeelig, Burckhard2023-08-172023-08-172023-07https://hdl.handle.net/11299/256042UROP Project conducted in the Seelig Lab under the direction of Burckhard Seelig. Polyhistidine (His) and Maltose Binding Protein (MBP) tagged proteins were successfully expressed in Escherichia coli. TEV protease cleavage and MBP-free cloning were pursued as strategies for obtaining pure, MBP-free protein variants for further research. A protocol for TEV cleavage was developed, and methods of subsequent purification or alternative cloning were explored.Polyhistidine (His) and Maltose Binding Protein (MBP) tagged proteins were successfully expressed in Escherichia coli. TEV protease cleavage and MBP-free cloning were pursued as strategies for obtaining pure, MBP-free protein variants for further research. A protocol for TEV cleavage was developed, and methods of subsequent purification or alternative cloning were explored.enBiochemistryTEV ProteaseFusion ProteinAffinity Tag CleavageCBSPresentation