Husband, Holly2024-04-302024-04-302023-07https://hdl.handle.net/11299/262852University of Minnesota M.S. thesis. July 2023. Major: Food Science. Advisor: Baraem Pam Ismail. 1 computer file (PDF); xiv, 110 pages.The relatively low abundance of 11S legumin in pea protein, coupled with the wide diversity in 7S vicilin to 11S legumin ratio among pea protein ingredients, are assumed contributors to pea protein’s inferior and inconsistent functionality and nutritional quality relative to soy protein. To improve the performance of pea protein ingredients in food and beverage applications, optimum protein profile must be identified. Therefore, this work followed a holistic approach to determine the impact of 7S/11S ratio on pea protein structure, functionality, and nutritional quality. Vicilin- and legumin-rich fractions were isolated and combined in different proportions to produce pea protein samples of varying 7S/11S ratios. For the first time, pea protein isolate was also enriched with 11S legumin to evaluate the impact of 11S abundance on functionality within an unfractionated protein matrix. Results revealed the isolated 7S vicilin had 6 fold higher gel strength and 5 fold higher emulsification capacity, but significantly lower nutritional quality, than the isolated 11S legumin. Despite having significantly higher sulfur-containing amino acids, high protein polymerization in the isolated 11S legumin contributed to the relatively low functionality. Further, fractionation induced unique changes to amino acid composition, resulting in significantly lower amino acid scores for isolated 7S vicilin and 11S legumin relative to pea protein isolate. Accordingly, 11S legumin enrichment of pea protein isolate did not improve functionality or nutritional quality. Nevertheless, this work contributed foundational knowledge that will provide direction for future studies aiming at devising strategies to improve the quality and consistency of pea protein ingredients.en7S vicilin/11S legumin ratiofunctional propertiesnutritional qualitypea proteinstructural characteristicsThesis or Dissertation