Jones, DebaratiJones, Robert S.2024-11-212024-11-212024-11-21https://hdl.handle.net/11299/267900Data supporting figures in https://doi.org/10.3390/dj12110366 1. File List A. Filename: DRUM_Ghose_JonesRS_Fig2A.csv Short description: Data for corresponding figure described in file name B. Filename: DRUM_Ghose_JonesRS_Fig2B.csv Short description: Data for corresponding figure described in file name C. Filename:DRUM_Ghose_JonesRS_Fig2C.csv Short description: Data for corresponding figure described in file name D. Filename:Figure DRUM_Ghose_JonesRS_Fig2D.csv Short description: Data for corresponding figure described in file name E. Filename:DRUM_Ghose_JonesRS_Fig3A.csv Short description: Data for corresponding figure described in file name F. Filename:DRUM_Ghose_JonesRS_Fig3B.csv Short description:Data for corresponding figure described in file nameAn alternative and understudied microbial mechanism that may influence demineralization is the microbially mediated ion exchange of Ca2+ and orthophosphate (Pi), which alters the saturation state of the mineral species within the surface enamel. There is a need to examine the ability of members of the oral microbiome to modulate Ca2+ and Pi, which control mineral solubility, in order to effectively evaluate mineralization therapies to improve oral health. (2) Methods: Pi uptake was measured using an ascorbic acid assay during a BHI liquid culture growth of Corynebacterium matruchotii and Streptococcus mutans for up to 20 h. The initial and endpoint medium Ca2+ levels were measured using ICP-OES. Bacterial cells were examined at different growth stages using DAPI/polyP binding emission at 525 nm to detect the presence of internalized macromolecules of polyphosphates (polyP) that could drive Pi uptake. (3) Results: C. matruchotii (p = 0.0061) substantially accumulated Pi (3.84 mmol/L), with a concomitant formation of polyP. In contrast, S. mutans did not take up Pi or accumulate polyP. No significant Ca2+ drawdown in the media was observed in either strain. (4) Conclusions: This study suggests that when examining the future efficacy of prevention technologies to improve, in vitro assays may consider including specific oral bacteria capable of substantial Pi uptake.oral; bacteria; calcium; phosphate; polyphosphateDataset