Menke, Jon R.2011-12-292011-12-292011-05https://hdl.handle.net/11299/119322University of Minnesota Ph.D. dissertation. May 2011. Major: Plant Pathology. Advisor: H. Corby Kistler. 1 computer file (PDF); xi, 211 pages.The plant pathogen F. graminearum (Gibberella zeae) presents a two-fold threat to farmers and consumers. Not only does this filamentous fungus cause the disease Fusarium head blight (FHB) that results in significant yield loss in infected grains, it also taints these grains with potent mycotoxins harmful to humans, animals, and plants alike. Equally alarming is the evidence that grain can appear to be physically sound while still being significantly contaminated with trichothecene mycotoxins. Tri12 encodes a predicted major facilitator superfamily transporter protein suggested to play a role in the export of trichothecene mycotoxins produced by the Fusarium species. However, the role of Tri12p in toxin sensitivity and plant pathogenicity of Fusarium graminearum was previously unknown. In this study, the correct intron positions for Tri12 in F. graminearum (FgTri12) were established using cDNA sequencing, EST data, and comparative genomics. Reverse genetics was used to establish that FgTri12 plays a role in self-protection and influences toxin production and virulence of the fungus in planta. To identify the subcellular location of FgTri12p during toxin production in culture, FgTri12p was tagged with eGFP. FgTri12p::eGFP was localized in small motile vesicles, the plasma membrane, and the lumen of vacuoles within fungal cells. Treatment of cells with latrunculin A resulted in the absence of motile vesicles labeled with FgTri12p::eGFP, suggesting their formation relies upon actin polymerization. To determine if FgTri12p co-localizes with enzymes involved in trichothecene metabolism, its cellular fate was compared with FgTri1p::eGFP, a fluorescently tagged oxygenase catalyzing a key intermediate step in trichothecene biosynthesis. While FgTri1p::eGFP initially localizes to small motile vesicles and later accumulates in the vacuole, during the period of initial trichothecene biosynthesis it is targeted to the periphery of intermediate sized vesicles, presumed to be the site of toxin synthesis. These results indicate FgTri12 plays a role in self-protection and influences toxin production and virulence of the fungus in planta. The interactions between F. graminearum and its hosts – wheat, rice, or barley – differ in disease severity and the levels of trichothecenes that accumulate in response to infection. The transcriptome of the fungus in rice and wheat was examined in order to identify genes expressed in planta. The hypothesis that fungal genes expressed in planta, but not during growth in culture, could include those that determine the plant infection phenotype was tested by reverse genetics: Four genes expressed exclusively in planta were deleted and two of these were determined to significantly alter disease phenotype. FGSG_03539, also called Tri9, is a previously uncharacterized gene in the major trichothecene biosynthetic gene cluster. A mutant with a tri9 deletion has attenuated virulence and lower trichothecene levels in wheat compared to wild type or a mutant strain complemented with the intact Tri9 gene. FGSG_11164 encodes a predicted trypsin protease and deletion of this gene also results in a small but significant reduction in pathogenicity toward wheat. The results demonstrate that a reverse genetic approach using in planta gene expression data may supplement forward genetic screens for identifying genes encoding virulence factors.en-USFusarium graminearumGene expressionReverse geneticsTri12TrichothecenesVirulencePlant PathologyThesis or Dissertation