Li, Chao2020-02-262020-02-262019-12https://hdl.handle.net/11299/211776University of Minnesota Ph.D. dissertation. December 2019. Major: Biochemistry, Molecular Bio, and Biophysics. Advisor: Carrie Wilmot. 1 computer file (PDF); viii, 123 pages.This thesis included my work on the crystallization, data analysis and phasing attempts regarding RcnR in complex with a ds-DNA molecule .RcnR is a transcription factor that regulates the homeostasis of cobalt and nickel in bacterial cells. We crystallized Escherichia coli RcnR with DNA that encompasses the DNA binding site. X-ray diffraction data were collected to 2.9 Å. The crystal belongs to space group P61/522, with unit cell parameters a = b = 73.65 Å, c= 153.77Å, α=β=90°, γ = 120°. The second and third part included my work on MauG and PqqB projects.enComplexcrystallizationDNAphasingRcnRThesis or Dissertation