Wei, GuanjuYang, Judy2022-05-092022-05-092022-05-09https://hdl.handle.net/11299/227295The images file contains the raw images collected using Nikon confocal microscope. The codes are used for processing these raw images to calculate the biofilm thickness. The experimental data file contains the experiment parameter and the data after processing.This dataset consists of the codes, experimental data, and raw images of the Pseudomonas putida biofilm development experiments. They are all collected in the Saint Anthony Falls Laboratory at the University of Minnesota. The codes are used for processing the raw images to calculate the biofilm thickness. The experimental data file contains the experiment parameters and the data after processing. The image file contains the raw images collected using the Nikon confocal microscope.CC0 1.0 UniversalBiofilmMicrofluidicsDatasethttps://doi.org/10.13020/afnk-kp31