McKenzie, Philip2016-12-192016-12-192016-08https://hdl.handle.net/11299/183291University of Minnesota M.S. thesis. August 2016. Major: Dentistry. Advisor: Scott McClanahan. 1 computer file (PDF); v, 64 pages.The purposes of this study are to; determine the length of calcium hydroxide medication most appropriate for killing a biofilm sourced from a clinical case treated with a regenerative endodontic procedure, and characterize the bacterial community found in an infected tooth with clinical features of those that would commonly be treated with regenerative endodontic procedures. Further studies are required to demonstrate unique characteristics of the microbiological community found in immature necrotic teeth with apical periodontitis. Within the limitations of this study, the author was unable to demonstrate that greater than 1 week of calcium hydroxide treatment resulted in any clinical benefit.enThesis or Dissertation