Warner, Gary Andrew2010-08-312010-08-312010-06https://hdl.handle.net/11299/93548University of Minnesota M.S. thesis. June 2010. Major: Dentistry. Advisor: Brent Evans Larson. 1 computer file (PDF); vi, 41 pages. Ill. (some col.)Introduction: Current literature suggests that the appearance of white spot lesions (WSLs) can be improved by treatment with casein phosphopeptides (CPP) and amorphous calcium phosphate (ACP). The aim of this study was to test the null hypothesis that the treatment of WSLs with CPP-ACP complexes produces no difference in the outcome of WSL regression when compared to controls regardless of whether or not microabrasion is used. Method: WSLs were artificially induced on 4 tooth sections obtained from each of 16 bovine central incisors, and then each section was randomly assigned to one of four groups. The three treatment groups were: CPP-ACP only group; a microabrasion only group, and a microabrasion and CPP-ACP group. The control group was neither CPP-ACP nor microabrasion. Quantitative light-induced fluorescence (QLF) was used to measure mineral content changes in WSLs immediately before (T1) and 2 weeks after treatment (T2). A two-within subject factor ANOVA was used to analyze the significance of any change from T1 to T2 in fluorescence, indicating mineral gain or loss. Results: There was a statistically significant gain in mineral content (p=0.0058) associated with the microabrasion technique, but no such gain for CPP-ACP (p=0.40). Conclusions: The null hypothesis was accepted. CPP-ACP complexes do not significantly improve the mineral content of bovine white spot lesions in vitro. Within the limitations of this in vitro study, the gains in mineral content seen with CPP-ACP were not statistically significant, and the gains in mineral content found when CPP-ACP was preceded by microabrasion were due to microabrasion, but not to the CPP-ACP complexes.en-USWhite spot lesions (WSLs)CPP-ACP groupMicroabrasionMineralDentistryEnamel lesions in vitroThesis or Dissertation