Heins, BradleyHuson, HeatherDechow, ChadAzwan Jaafar, Mohd2022-04-272022-04-272022-04-27https://hdl.handle.net/11299/227134Our study evaluated two 3-breed rotational crossbred dairy cattle populations. ProCROSS is a product of crossbreeding Viking Red, Holstein, and Montbeliarde whereas Grazecross consists of Viking Red, Normande, and Jersey. The rotational crossbreeding system capitalizes on heterosis with each generation and the specific breeds used target trait improvement and development for certain environments.The effect of ancestry and use of reference populations in determining ancestry in ProCROSS and Grazecross were analyzed using genomic and pedigree-based estimations.Our study provides evidence of the importance of identifying the most appropriate reference animals and the effect of ancestry composition on ProCROSS and Grazecross dairy cattle performance. This work is supported by Organic Agriculture Research and Extension Initiative [grant no. 2016-51300-25862/project accession no. 1010366] from the USDA National Institute of Food and Agriculture.CC0 1.0 Universalgenotypegenomicsdairy cattleDataset