Wei, Lai2021-04-302021-04-302021https://hdl.handle.net/11299/219533Faculty advisor: Dr. Yue ChenThe Chen lab developed a new technique that uses quantitative proteomics approach for site-specific ubiquitination stoichiometry analysis, named the Isotopically Balanced Quantification of Ubiquitination (IBAQ-Ub), which is a method that quantifies the absolute abundance of ubiquitination. IBAQ-Ub and in vitro ubiquitination enzymatic assays were conducted to characterize the site-specific ubiquitination dynamics and linkage specificity of RNF168 in DDR(DNA-damage-response).enCollege of Biological SciencesDepartment of Biochemistry, Molecular Biology and BiophysicsPresentation