This readme.txt file was generated on 2023-05-30 by Brooke R. Kern ------------------- GENERAL INFORMATION ------------------- 1. Title of Dataset: Direct tracking of pollen with quantum dots reveals surprising uniformity in dispersal distance across eleven populations of an annual plant 2. Author Information Principal Investigator Contact Information Name: Brooke Kern Institution: University of Minnesota Email: kern0193@umn.edu ORCID: https://orcid.org/0000-0001-6872-592X Associate or Co-investigator Contact Information Name: Lauren N. Carley Institution: University of Minnesota ORCID: https://orcid.org/0000-0003-1911-7025 Associate or Co-investigator Contact Information Name: David A. Moeller Institution: University of Minnesota ORCID: https://orcid.org/0000-0002-6202-9912 3. Date published or finalized for release: 2023-05-30 4. Date of data collection: 2021 - 2022 5. Geographic location of data collection (where was data collected?): Kern County, CA 6. Information about funding sources that supported the collection of the data: National Science Foundation (DEB-1754246 and DEB-1754026 to D.A.M.) and an award from the Bell Museum of Natural History (U. of Minnesota) to B.R.K. 7. Overview of the data (abstract): Pollen movement is a crucial component of dispersal in seed plants. We used quantum dot pollen labeling, a new technique that overcomes past limitations, to evaluate the spatial scale of pollen dispersal and its relationship with conspecific density within eleven populations of Clarkia xantiana ssp. xantiana, a bee pollinated, annual plant. We used experimental arrays in two years to track pollen movement across distances of 5 – 35 m within nine populations and across distances of 10 – 70 m within two additional populations. We tested for distance decay of pollen dispersal and whether conspecific density modulated dispersal distance. We also asked whether dispersal kernels varied among populations across an environmentally complex landscape. We did not observe a decline in labeled pollen receipt with distance over 35 m within eight of nine populations or over 70 m within either of two populations. Pollen receipt increased with conspecific density. Overall, dispersal kernels were consistent across populations. -------------------------- SHARING/ACCESS INFORMATION -------------------------- 1. Licenses/restrictions placed on the data: CC 1.0 Universal 2. Links to publications that cite or use the data: Kern, Brooke R., Carley, Lauren N., and Moeller, David A. 2023. Direct tracking of pollen with quantum dots reveals surprising uniformity in dispersal distance across eleven populations of an annual plant. American Journal of Botany. https://doi.org/10.1002/ajb2.16201 3. Was data derived from another source? No. 4. Terms of Use: Data Repository for the U of Minnesota (DRUM) By using these files, users agree to the Terms of Use. https://conservancy.umn.edu/pages/drum/policies/#terms-of-use --------------------- DATA & FILE OVERVIEW --------------------- 1. File List A. Filename: Datafile_Density_MidRange.csv Short description: This file contains counts of conspecific flowering plants and total conspecific flowers within a 0.5 m radius of each pollen recipient in the mid-range (5 - 35 m) arrays. B. Filename: Datafile_Pollen_MidRange.csv Short description: This file contains pollen counts for each recipient stigma in the mid-range arrays. C. Filename: Datafile_Density_LongRange.csv Short description: This file contains counts of conspecific flowering plants and total conspecific flowers within a 0.5 m radius of each pollen recipient in the two long-range (10 - 70 m) arrays, as well as the distance to the nearest neighbor flowering conspecific for each pollen recipient. D. Filename: Datafile_Pollen_LongRange.csv Short description: This file contains pollen counts for each recipient stigma in the long-range arrays. E. Filename: Datafile_Pollen_ShortRange.csv Short description: This file contains pollen counts for each recipient stigma in the short-range (0.5 - 5 m) arrays. 2. Relationship between files: For the mid- and long-range arrays, the density at each recipient plant can be matched to the pollen counts using the locationID column. -------------------------- METHODOLOGICAL INFORMATION -------------------------- 1. Description of methods used for collection/generation of data: We set up experimental arrays using cut stems of Clarkia xantiana ssp. xantiana within natural populations. In each array, specific plants were used as pollen donors and had pollen labeled with fluorescent quantum dots. We collected stigmas from recipient plants at set distances from the donors and counted the number of labeled pollen grains on each stigma. We also measured conspecific density of natural plants in a 0.5 m radius around each recipient plant. For full methods, see citation: Kern, Brooke R., Carley, Lauren N., and Moeller, David A. 2023. Direct tracking of pollen with quantum dots reveals surprising uniformity in dispersal distance across eleven populations of an annual plant. American Journal of Botany. https://doi.org/10.1002/ajb2.16201 2. Methods for processing the data: These are the raw data. 3. Instrument- or software-specific information needed to interpret the data: None 4. Standards and calibration information, if appropriate: N/A 5. Environmental/experimental conditions: Experiments were conducted under natural field conditions. 6. Describe any quality-assurance procedures performed on the data: 7. People involved with sample collection, processing, analysis and/or submission: Authors listed above ---------------------------------------------------------------- DATA-SPECIFIC INFORMATION FOR: Datafile_Density_MidRange.csv ---------------------------------------------------------------- 1. Number of variables: 7 2. Number of cases/rows: 270 3. Missing data codes: No missing data 4. Variable List A. Name: locationID Description: Combination of site, distance, and direction to give each location with a recipient plant a unique ID B. Name: direction Description: "Spoke" of the circular array where the recipient plant was located, with "spoke A" in the 12 o'clock position (see Fig. 1C in the related article for a diagram of the array layout) C. Name: distance Description: Distance (in m) from the pollen donors D. Name: numFloweringPlants Description: Number of conspecific flowering plants within a 0.5m radius of the recipient plant E. Name: numFlowers Description: Number of conspecific flowers within a 0.5m radius of the recipient plant F. Name: site Description: Population where the stigma was collected. BC: Bodfish Crest BG: Black Gulch BR: Borel Road C3: Camp 3 (written as CP3 in the article) FR: Freeway Ridge OSR: Old State Road S8: Site 8 SAW: Sawmill Road East (written as SMRE in the article) US: Upper Sawmill/Sawmill Road West (written as SMRW in the article) H. Name: date Description: Date density was measured (the first day plants were placed in the experimental array) ---------------------------------------------------------------- DATA-SPECIFIC INFORMATION FOR: Datafile_Pollen_MidRange.csv ---------------------------------------------------------------- 1. Number of variables: 13 2. Number of cases/rows: 963 3. Missing data codes: No missing data 4. Variable List A. Name: dateCount Description: Date the pollen was counted from the top of the slide (i.e., the side with the coverslip) B. Name: dateCollect Description: Date the stigma was collected in the field C. Name: dayTrial Description: Day of the experiment (2, 3, or 4) the stigma was collected D. Name: site Description: Population where the stigma was collected BC: Bodfish Crest BG: Black Gulch BR: Borel Road C3: Camp 3 (written as CP3 in the article) FR: Freeway Ridge OSR: Old State Road S8: Site 8 SAW: Sawmill Road East (written as SMRE in the article) US: Upper Sawmill/Sawmill Road West (written as SMRW in the article) E. Name: direction Description: "Spoke" of the circular array where the recipient plant was located, with "spoke A" in the 12 o'clock position (see Fig. 1C in the related article for a diagram of the array layout) F. Name: distance Description: Distance (in m) from the pollen donors G. Name: stigma Description: Arbitrary code used to give each stigma a unique ID H. Name: locationID Description: Combination of site, distance, and direction to give each location with a recipient plant a unique ID I. Name: stigmaID Description: Combination of site, distance, direction, and stigma number to give each stigma a unique ID J. Name: hasPollen Description: Does the stigma have any pollen on it (labeled and/or unlabeled)? Yes (y) or no (n) K. Name: topLabeledCount Description: Number of labeled (fluorescent) pollen grains counting from the top of the slide (i.e., the side with the coverslip). Can be added to backLabeledCount to get the total number of labeled pollen grains L. Name: backLabeledCount Description: Number of labeled (fluorescent) pollen grains counting from the bottom of the slide (i.e., the side opposite the coverslip). Can be added to topLabeledCount to get the total number of labeled pollen grains M. Name: dateCountBack Description: Date the pollen was counted from the back of the slide ---------------------------------------------------------------- DATA-SPECIFIC INFORMATION FOR: Datafile_Density_LongRange.csv ---------------------------------------------------------------- 1. Number of variables: 8 2. Number of cases/rows: 52 3. Missing data codes: No missing data 4. Variable List A. Name: site Description: Population where the array was set up CG: China Gardens LCW: Lucas Creek West B. Name: distance Description: Distance (in m) from the pollen donors C. Name: letter Description: Letter used to distinguish different recipients at the same distance D. Name: locationID Description: Combination of site, distance, and letter to give each location with a recipient plant a unique ID E. Name: numFlowers Description: Number of conspecific flowers within a 0.5m radius of the recipient plant F. Name: numFloweringPlants Description: Number of conspecific flowering plants within a 0.5m radius of the recipient plant G. Name: distNearestPlant Description: Distance (in m) to the nearest flowering conspecific from the recipient plant H. Name: date Description: Date density was measured (the first day plants were placed in the experimental array) ---------------------------------------------------------------- DATA-SPECIFIC INFORMATION FOR: Datafile_Pollen_LongRange.csv ---------------------------------------------------------------- 1. Number of variables: 12 2. Number of cases/rows: 251 3. Missing data codes: No missing data 4. Variable List A. Name: dateCount Description: Date the pollen was counted B. Name: dateCollect Description: Date the stigma was collected in the field C. Name: dayTrial Description: Day of the experiment (2, 3, or 4) the stigma was collected D. Name: site Description: Population where the stigma was collected CG: China Gardens LCW: Lucas Creek West E. Name: letter Description: Letter used to distinguish different recipients at the same distance F. Name: distance Description: Distance (in m) from the pollen donors G. Name: stigma Description: Arbitrary code used to give each stigma a unique ID H. Name: locationID Description: Combination of site, distance, and letter to give each location with a recipient plant a unique ID I. Name: stigmaID Description: Combination of site, distance, letter, and stigma number to give each stigma a unique ID J. Name: hasPollen Description: Does the stigma have any pollen on it (labeled and/or unlabeled)? Yes (y) or no (n) K. Name: topLabeledCount Description: Number of labeled (fluorescent) pollen grains counting from the top of the slide (i.e., the side with the coverslip). Can be added to backLabeledCount to get the total number of labeled pollen grains L. Name: backLabeledCount Description: Number of labeled (fluorescent) pollen grains counting from the bottom of the slide (i.e., the side opposite the coverslip). Can be added to topLabeledCount to get the total number of labeled pollen grains ---------------------------------------------------------------- DATA-SPECIFIC INFORMATION FOR: Datafile_Pollen_ShortRange.csv ---------------------------------------------------------------- 1. Number of variables: 10 2. Number of cases/rows: 53 3. Missing data codes: No missing data 4. Variable List A. Name: dateCount Description: Date the pollen was counted B. Name: dateCollect Description: Date the stigma was collected in the field C. Name: dayTrial Description: Day of the experiment (2, 3, or 4) the stigma was collected D. Name: site Description: Population where the stigma was collected. C3: Camp 3 SR: Sawmill Road East E. Name: distance Description: Distance (in m) from the pollen donors F. Name: stigma Description: Arbitrary code used to give each stigma a unique ID G. Name: stigmaID Description: Combination of site, distance, and stigma number to give each stigma a unique ID J. Name: hasPollen Description: Does the stigma have any pollen on it (labeled and/or unlabeled)? Yes (y) or no (n) K. Name: topLabeledCount Description: Number of labeled (fluorescent) pollen grains counting from the top of the slide (i.e., the side with the coverslip). Can be added to backLabeledCount to get the total number of labeled pollen grains L. Name: backLabeledCount Description: Number of labeled (fluorescent) pollen grains counting from the bottom of the slide (i.e., the side opposite the coverslip). Can be added to topLabeledCount to get the total number of labeled pollen grains