This readme.txt file was generated on 2021-10-08 by Wesley J. Glisson ------------------- GENERAL INFORMATION ------------------- 1. Title of Dataset Complete Data for: Desiccation tolerance of the invasive alga starry stonewort (Nitellopsis obtusa) as an indicator of overland spread risk 2. Author Information Principal Investigator Contact Information Name: Wesley Glisson Institution: University of Minnesota Address: 135 Skok Hall, 2003 Upper Buford Circle, St Paul MN 55108 Email: wjglisson@gmail.com ORCID: https://orcid.org/0000-0002-2540-3696 Associate or Co-investigator Contact Information Name: Carli K. Wagner Institution: University of Minnesota Address: 135 Skok Hall, 2003 Upper Buford Circle, St Paul MN 55108 Associate or Co-investigator Contact Information Name: Michael R. Verhoeven Institution: University of Minnesota Address: 135 Skok Hall, 2003 Upper Buford Circle, St Paul MN 55108 ORCID: https://orcid.org/0000-0002-6340-9490 Associate or Co-investigator Contact Information Name: Ranjan Muthukrishnan Institution: Boston University Address: 5 Cummington Mall, Boston, MA 02215 ORCID: https://orcid.org/0000-0002-7001-6249 Associate or Co-investigator Contact Information Name: Rafael Contreras-Rangel Institution:University of Minnesota Address: 135 Skok Hall, 2003 Upper Buford Circle, St Paul MN 55108 Associate or Co-investigator Contact Information Name: Daniel J. Larkin Institution: University of Minnesota Address: 135 Skok Hall, 2003 Upper Buford Circle, St Paul MN 55108 Email: djlarkin@umn.edu ORCID: https://orcid.org/0000-0001-6378-0495 3. Date of data collection: Data collected 2017-08-08 through 2017-12-08 4. Geographic location of data collection (where was data collected?): Minnesota Aquatic Invasive Species Research Center (MAISRC) Containment Laboratory (MCL), University of Minnesota, Saint Paul, Minnesota. 5. Information about funding sources that supported the collection of the data: Funding for this project was provided by the Minnesota Environment and Natural Resources Trust Fund as recommended by the Minnesota Aquatic Invasive Species Research Center (MAISRC) and the Legislative-Citizen Commission on Minnesota Resources (LCCMR). -------------------------- SHARING/ACCESS INFORMATION -------------------------- 1. Licenses/restrictions placed on the data: Attribution-ShareAlike 3.0 United States 2. Links to publications that cite or use the data: Glisson WJ, Wagner CK, Verhoeven MR, Muthukrishnan R, Contreras-Rangel R, Larkin DJ (2020) Desiccation tolerance of the invasive alga starry stonewort (Nitellopsis obtusa) as an indicator of overland spread risk. Journal of Aquatic Plant Management 58: 7–18 Journal of Aquatic Plant Management: http://www.apms.org/wp/wp-content/uploads/japm-58-01-7.pdf Minnesota Aquatic Invasive Species Research Center (MAISRC): https://drive.google.com/file/d/1DkUMHi4z5K6IHtZaqvsNd1DxwsGqJK6p/view 3. Links to other publicly accessible locations of the data: NA 4. Links/relationships to ancillary data sets: NA 5. Was data derived from another source? No 6. Recommended citation for the data: Glisson, Wesley J.; Wagner, Carli K.; Verhoeven, Michael R.; Muthukrishnan, Ranjan; Contreras-Rangel, Rafael; Larkin, Daniel J.. (2021). Complete Data for: Desiccation tolerance of the invasive alga starry stonewort (Nitellopsis obtusa) as an indicator of overland spread risk. Retrieved from the Data Repository for the University of Minnesota, https://doi.org/10.13020/28th-gy94. --------------------- DATA & FILE OVERVIEW --------------------- 1. File List A. Filename: Nobtusa_desiccationData_augustExperiment_fragments.csv Short description: Data for Nitellopsis obtusa fragments from the outdoor desiccation experiment conducted August 2017. B. Filename: Nobtusa_desiccationData_augustExperiment_bulbilsTZ_photos.zip Short description: A compressed folder containing photographs of the front and back of all replicate experimental bulbils evaluated for viability via tetrazolium staining from the outdoor desiccation experiment conducted August 2017. Photos are located in individual folders for each treatment and controls. C. Filename: Nobtusa_desiccationData_octoberExperiment_fragments.csv Short description: Data for Nitellopsis obtusa fragments from the laboratory desiccation experiment conducted October 2017. D. Filename: Nobtusa_desiccationData_octoberExperiment_bulbils.csv Short description: Data for Nitellopsis obtusa bulbils from the laboratory desiccation experiment conducted October 2017. E. Filename: Nobtusa_desiccationData_octoberExperiment_bulbilWeights.csv Short description: Data for Nitellopsis obtusa bulbil weights from the laboratory desiccation experiment conducted October 2017. F. Filename: Nobtusa_desiccationData_octoberExperiment_tempRH.csv Short description: Temperature and relative humidity recorded during the Nitellopsis obtusa laboratory desiccation experiment conducted October 2017. G. Filename: Nobtusa_desiccationData_octoberExperiment_fragmentsClumps_photos.zip Short description: A compressed folder containing photographs of all control and 24-hr replicate jars used for physical assessment of Nitellopsis obtusa viability from the laboratory desiccation experiment conducted October 2017. Photos are located in individual folders for each fragment type (single fragment, small clump, and large clump), and subsequently into folders for treatment and control replicates. 2. Relationship between files: NA 3. Additional related data collected that was not included in the current data package: NA 4. Are there multiple versions of the dataset? No -------------------------- METHODOLOGICAL INFORMATION -------------------------- 1. Description of methods used for collection/generation of data: For a description of the methods used for collection/generation of data please see: Glisson WJ, Wagner CK, Verhoeven MR, Muthukrishnan R, Contreras-Rangel R, Larkin DJ (2020) Desiccation tolerance of the invasive alga starry stonewort (Nitellopsis obtusa) as an indicator of overland spread risk. Journal of Aquatic Plant Management 58: 7–18 Please see SHARING/ACCESS INFORMATION #2 above for links to the article. 2. Methods for processing the data: Original data was collected on paper datasheets and then transcribed into a digital format (MS Excel). 3. Instrument- or software-specific information needed to interpret the data: NA 4. Standards and calibration information, if appropriate: NA 5. Environmental/experimental conditions: For a description of the environmental/experimental conditions please see: Glisson WJ, Wagner CK, Verhoeven MR, Muthukrishnan R, Contreras-Rangel R, Larkin DJ (2020) Desiccation tolerance of the invasive alga starry stonewort (Nitellopsis obtusa) as an indicator of overland spread risk. Journal of Aquatic Plant Management 58: 7–18 Please see SHARING/ACCESS INFORMATION #2 above for links to the article. 6. Describe any quality-assurance procedures performed on the data: All data were checked for accuracy and consistency upon entry and analysis. 7. People involved with sample collection, processing, analysis and/or submission: All authors contributed to these steps. ---------------------------------------------------------------------------------------- DATA-SPECIFIC INFORMATION FOR: Nobtusa_desiccationData_augustExperiment_fragments.csv ---------------------------------------------------------------------------------------- 1. Number of variables: 9 2. Number of cases/rows: 270 3. Missing data codes: Code/symbol: NA Definition: Data were either not applicable (e.g., no dry-weight measurements for wet [non-desiccated] controls), or not collected because the sample was lost or destroyed during desiccation (only applies to desiccation treatments). 4. Variable List A. Name: ident Description: Unique numeric identifier for each sample in the dataset. B. Name: treatment_hour Description: The desiccation time treatement for each sample. For example, 1h = 1-hour desiccation time treatment; control = wet (non-desiccated) controls. C. Name: treatment_type Description: The treatement type for each sample. fr = Single fragment sc = Small clump lc = Large clump D. Name: treatment_ID Description: The combination of treatment_hour, treatment_type, and the replicate number (1-10) for each sample. E. Name: pvc_frame Description: The frame upon which the sample was placed, either A or B. F. Name: line_number Description: The line (between frames) on which the sample was placed. G. Name: initial_weight_g Description: The intial wet weight (in grams) of the sample. H. Name: dry_weight_g Description: The dried weight following desiccation (in grams) of the sample. I. Name: rehydrated_weight_g Description: The rehydrated weight (in grams) of the sample. -------------------------------------------------------------------------------------- DATA-SPECIFIC INFORMATION FOR: Nobtusa_desiccationData_octoberExperiment_fragments.csv -------------------------------------------------------------------------------------- 1. Number of variables: 12 2. Number of cases/rows: 455 3. Missing data codes: Code/symbol: NA Definition: Data were either not applicable (e.g., no dry-weight measurements for wet [non-desiccated] controls), or not collected because the sample was lost or destroyed during desiccation (only applies to desiccation treatments). 4. Variable List A. Name: line_order Description: Order of each sample on the string between pvc frames. B. Name: treatment_hour Description: The desiccation time treatement for each sample. For example, 1h = 1-hour desiccation time treatment; control = wet (non-desiccated) controls; controlD = oven-dried controls. C. Name: time_hr Description: The desiccation time, in hours, for each sample. D. Name: time_min Description: The desiccation time, in minutes, for each sample. E. Name: treatment_type Description: The treatement type for each sample. fr = Single fragment sc = Small clump lc = Large clump F. Name: replicate_number Description: The replicate number for each sample, for each fragment type. G. Name: treatment_ID Description: The combination of treatment_hour, treatment_type, and the replicate number (1-10) for each sample. H. Name: pvc_frame Description: The frame upon which the sample was placed, either A or B. I. Name: line_number Description: The line (between frames) on which the sample was placed. J. Name: initial_weight_g Description: The intial wet weight (in grams) of the sample. K. Name: dry_weight_g Description: The dried weight following desiccation (in grams) of the sample. L. Name: rehydrated_weight_g Description: The rehydrated weight (in grams) of the sample. -------------------------------------------------------------------------------------- DATA-SPECIFIC INFORMATION FOR: Nobtusa_desiccationData_octoberExperiment_bulbils.csv -------------------------------------------------------------------------------------- 1. Number of variables: 7 2. Number of cases/rows: 351 3. Missing data codes: Code/symbol: "tbd" Definition: Until a replicate jar contained a sprouted bulbil, the name of the replicate jar was not recorded (i.e., recorded as "tbd"). All replicate jars were recorded on the final day of the experiment for the associated desiccation time treatment. Code/symbol: NA Definition: Data were not applicable. This applies only to the last two columns of the data ("unsprouted_including_unrecovered" and "unrecovered"), which were exclusively recorded on the final day of the experiment for each desiccation time treatment. 4. Variable List A. Name: date Description: The date of observation (format: MM/DD/YYYY). B. Name: tank Description: The tank (1,2, or 3) in which each replicate jar was located. C. Name: treatment_hour Description: The desiccation time treatement for each sample. For example, 1h = 1-hour desiccation time treatment; control = wet (non-desiccated) controls; controlD = oven-dried controls. D. Name: replicate_jar Description: The replicate jar number. E. Name: sprouted_count Description: The count of sprouted bubils in a given replicate jar for a given date. F. Name: unsprouted_including_unrecovered Description: The number of bulbils that did not sprout by the end of the experiment, including the number of bulbils that could not be recovered. G. Name: unrecovered Description: At the conclusion of the experiment, the number of bulbils that were not recovered. ------------------------------------------------------------------------------------------ DATA-SPECIFIC INFORMATION FOR: Nobtusa_desiccationData_octoberExperiment_bulbilWeights.csv ------------------------------------------------------------------------------------------ 1. Number of variables: 3 2. Number of cases/rows: 13 3. Missing data codes: Code/symbol: NA Definition: Data were not applicable; no dried weight for the wet (non-desiccated) controls. 4. Variable List A. Name: treatment_hour Description: The desiccation time treatement for each sample. For example, 1h = 1-hour desiccation time treatment. control = wet (non-desiccated) controls. controlD = oven-dried controls. B. Name: initial_weight_g Description: The initial wet weight (in grams) of all bulbils combined for each treatment. C. Name: dried_weight_g Description: The dried weight following desiccation (in grams) of all bulbils combined for each treatment. ------------------------------------------------------------------------------------------ DATA-SPECIFIC INFORMATION FOR: Nobtusa_desiccationData_octoberExperiment_tempRH.csv ------------------------------------------------------------------------------------------ 1. Number of variables: 4 2. Number of cases/rows: 26 3. Missing data codes: None 4. Variable List A. Name: date Description: The date of observation (format: MM/DD/YYYY). B. Name: time Description: The observation time. C. Name: temp_F Description: Air temperature in degrees Fahrenheit. D. Name: relative_humidity Description: Relative humidity (%).