This readme.txt file was generated on <2025 by Recommended citation for the data: ------------------- GENERAL INFORMATION ------------------- 1. Title of Dataset: An Investigation of Dasatinib and Quercetin as Enhancers of Myofiber Regeneration in DMD 2. Author Information Principal Investigator Contact Information Name: Christina Pacak Institution: University of Minnesota Address: WMBB 4-188, 2101 6th Street SE, Minneapolis, MN, 55455 Email: cpacak@umn.edu ORCID: 0000-0002-7430-124X Associate or Co-investigator Contact Information Name: Venessa Agyapong Institution: University of Minnesota Address: 2101 6th Street SE, Minneapolis, MN, 55455 Email: agyap006@umn.edu ORCID: Associate or Co-investigator Contact Information Name: Jennifer Tinklenberg Institution: University of Minnesota Address: 2101 6th Street SE, Minneapolis, MN, 55455 Email: tinkl037@umn.edu ORCID: 0000-0002-7895-4950 3. Date published or finalized for release: 20251129 4. Date of data collection (single date, range, approximate date): 20250801 - 20251127 5. Geographic location of data collection (where was data collected?): University of Minnesota Twin Cities, Minneapolis, MN, 55455 6. Information about funding sources that supported the collection of the data: University of Minnesota’s Office of Undergraduate Research 7. Overview of the data (abstract): Ineffective myofiber repair mechanisms in Duchenne's Muscular Dystrophy (DMD) result in progressive muscle tissue degeneration. DMD aligns with 8 of the 12 hallmarks of aging, with the presence of senescent cells and stem cell exhaustion severely impairing regenerative capacity. As muscle cells are progressively lost and replaced by fat deposits and fibrosis, secondary mechanisms of the disease give rise to phenotypes associated with premature aging that worsen over time. This study aims to improve regenerative capacity in DMD mouse myofibers by administering the senolytics Dasatinib and Quercetin (D+Q). D+Q was administered to mice at 4 weeks of age for a period of 21 weeks through oral gavage. Mice were sacrificed at 25 weeks of age, and the Flexor Digitorum Brevis (FDB) muscle myofibers will be isolated and cultured to observe the satellite cell migration and proliferation patterns for 48 hrs. Cell counts and migration distance calculations were performed at 24 hrs and 48 hrs compared to healthy controls. Fibers were fixed at 48 hrs for immunostaining of satellite cell markers, ATPB, and H2AX. Fibers from D+Q treated mdx mice did not have significant differences in the number of cells that migrated away from the fibers compared to their respective untreated mdx mice. This study is ongoing, and the fibers are being further evaluated for differences in satellite cell content using immunofluorescence markers. -------------------------- SHARING/ACCESS INFORMATION -------------------------- 1. Licenses/restrictions placed on the data: CC BY-NC-ND 4.0 2. Links to publications that cite or use the data: 3. Was data derived from another source? No 4. Terms of Use: Data Repository for the U of Minnesota (DRUM) By using these files, users agree to the Terms of Use. https://conservancy.umn.edu/pages/policies/#drum-terms-of-use --------------------- DATA & FILE OVERVIEW --------------------- 1. File List A. Filename: D+Q Satellite Cell Counts.csv Short description: Preliminary satellite cell counts B. Filename: An Investigation of Dasatinib and Quercetin as Enhancers of Myofiber Regeneration in DMD.pdf Short description: Poster presentation 2. Relationship between files: File contains the raw data used to generate the figure in the poster as well as the statistical values generated. -------------------------- METHODOLOGICAL INFORMATION -------------------------- 1. Description of methods used for collection/generation of data: Mice were administered Dasatinib (5 mg/kg) and Quercetin (50 mg/kg), dissolved in 10% polyethylene glycol 400, by gavage twice a week. Flexor digitorum brevis (FDB) muscles were harvested from 25 week old mice, then placed in 0.2% w/v collagenase solution to digest the ECM. Myofibers were then isolated by agitating FDB muscles in 10% HS. Myofibers were then cultured in a high serum environment to encourage satellite cell proliferation. Photos were taken at 24 and 48 hours of culturing. Cells were then fixed, then stored for further analysis. 2. Methods for processing the data: Photos of cultured fibers taken at 24 and 48 hour time were alanyzed for the number of satellite cells that migrated from the cells. For future analysis of Immunofluroecnce photos, ImageJ will be utilized to analyze fluorecnence intensities and proportion of MyoD+ satellite cells. 3. Instrument- or software-specific information needed to interpret the data: One-way ANOVA followed by post-hob(Tukey) testDunnett’s was performed using GraphPad Prism version 10.0.0 for Windows, GraphPad Software, Boston, Massachusetts USA, www.graphpad.com 4. Standards and calibration information, if appropriate: NA 5. Environmental/experimental conditions: NA 6. Describe any quality-assurance procedures performed on the data: NA 7. People involved with sample collection, processing, analysis and/or submission: Sample collection was carried out by Venessa Agyapong and Jennifer Tinklenberg Data for correlations was generated by Venessa Agyapong Analysis was conducted by Venessa Agyapong