DNA sequencing data of genomic DNA from Methanococcus maripaludis S2 suppressor strains that relies on Fdh2 when formate is the sole electron donor

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Collection period

2022-08-01
2022-13-01

Date completed

2022-10-06

Date updated

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Title

DNA sequencing data of genomic DNA from Methanococcus maripaludis S2 suppressor strains that relies on Fdh2 when formate is the sole electron donor

Published Date

2023-10-05

Author Contact

Costa, Kyle C.
kcosta@umn.edu

Type

Dataset
Genomics Data

Abstract

The data contains the Illumina Sequencing Reads of genome samples from Methanococcus maripaludis S2 suppressor mutant strains. These mutant strains has genomic deletion of both homologs of formate dehydrogenase (Fdh) and complemented with fdh2 homolog expressed from replicative vector. These strains only grew after prolonged incubations when suppressor mutations arise. These genome sequencing data revealed the gene mutations in these fdh2* suppressor strains.

Description

Data contains the Illumina Sequencing reads for genome sequencing of Methanococcus maripaludis suppressor strains that rely on Fdh2 for growth when formate is the sole electron donor.

Referenced by

Mohd Farid Abdul Halim, Dallas R Fonseca, Thomas D Niehaus, Kyle C Costa. (2023). Functionally redundant formate dehydrogenases enable formate-dependent growth in Methanococcus maripaludis. bioRxiv 2023.05.09.540023
https://doi.org/10.1101/2023.05.09.540023

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Funding information

U.S. Department of Energy, Office of Science, Basic Energy Sciences under grant number DE-SC0019148

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Suggested citation

Abdul Halim, Mohd Farid; Costa, Kyle C.; Fonseca, Dallas R.; Niehaus, Thomas. (2023). DNA sequencing data of genomic DNA from Methanococcus maripaludis S2 suppressor strains that relies on Fdh2 when formate is the sole electron donor. Retrieved from the Data Repository for the University of Minnesota (DRUM), https://doi.org/10.13020/fjgg-j094.

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Description
Size
FH1_S187_R1_001.fastq.gz
DNA sequencing for wild-type strain forward direction
(60.43 MB)

FH1_S187_R2_001.fastq.gz
DNA sequencing for wild-type strain reverse direction
(64.25 MB)

FH2_S188_R1_001.fastq.gz
Raw DNA sequencing data for fdh2* strain culture #1 - forward direction
(61.43 MB)

FH2_S188_R2_001.fastq.gz
Raw DNA sequencing data for fdh2* strain culture #1 - reverse direction
(64.55 MB)

FH3_S145_R1_001.fastq.gz
Raw DNA sequencing data for fdh2* strain culture #2 - forward direction
(105.41 MB)

FH3_S145_R2_001.fastq.gz
Raw DNA sequencing data for fdh2* strain culture #2 - reverse direction
(113.73 MB)

FH4_S146_R1_001.fastq.gz
Raw DNA sequencing data for fdh2* strain culture #3 - forward direction
(86.76 MB)

FH4_S146_R2_001.fastq.gz
Raw DNA sequencing data for fdh2* strain culture #3 - reverse direction
(93.26 MB)

FH5_S147_R1_001.fastq.gz
Raw DNA sequencing data for fdh2* strain culture #4 - forward direction
(91.54 MB)

FH5_S147_R2_001.fastq.gz
Raw DNA sequencing data for fdh2* strain culture #4 - reverse direction
(99.15 MB)

FH6_S148_R1_001.fastq.gz
Raw DNA sequencing data for fdh2* strain culture #5 - forward direction
(102.63 MB)

FH6_S148_R2_001.fastq.gz
Raw DNA sequencing data for fdh2* strain culture #5 - reverse direction
(111.04 MB)

FH7_S149_R1_001.fastq.gz
Raw DNA sequencing data for fdh2* strain culture #6 - forward direction
(91.2 MB)

FH7_S149_R2_001.fastq.gz
Raw DNA sequencing data for fdh2* strain culture #6 - reverse direction
(98.74 MB)

FH8_S150_R1_001.fastq.gz
Raw DNA sequencing data for fdh2* strain culture #7 - forward direction
(99.45 MB)

FH8_S150_R2_001.fastq.gz
Raw DNA sequencing data for fdh2* strain culture #7 - reverse direction
(107.23 MB)

FH9_S151_R1_001.fastq.gz
Raw DNA sequencing data for fdh2* strain culture #8 - forward direction
(88.45 MB)

FH9_S151_R2_001.fastq.gz
Raw DNA sequencing data for fdh2* strain culture #8 - reverse direction
(92.89 MB)

FH10_S152_R1_001.fastq.gz
Raw DNA sequencing data for fdh2* strain culture #9 - forward direction
(97.92 MB)

FH10_S152_R2_001.fastq.gz
Raw DNA sequencing data for fdh2* strain culture #9 - reverse direction
(105.69 MB)

FH11_S153_R1_001.fastq.gz
Raw DNA sequencing data for fdh2* strain culture #10 - forward direction
(97.76 MB)

FH11_S153_R2_001.fastq.gz
Raw DNA sequencing data for fdh2* strain culture #10 - reverse direction
(105.28 MB)

FH12_S154_R1_001.fastq.gz
Raw DNA sequencing data for fdh2* strain culture #11 - forward direction
(96.39 MB)

FH12_S154_R2_001.fastq.gz
Raw DNA sequencing data for fdh2* strain culture #11 - reverse direction
(104.8 MB)

SNP.geneious
Compiled data from all the strains sequencing analysis indicating the SNP mutations identified.
(2.42 GB)

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