Evaluation of the antiimicrobial activity of a new endodontic irrigant in a direct contact test using next generation sequencing

Abstract

The aim of this study was to test the antimicrobial activity of Triton against 4.4% sodium hypochlorite utilizing a mature multispecies biofilm. A direct contact test was conducted to compare the bacterial DNA removal by distilled water (negative control), sodium hypochlorite (NaOCl), and Triton (Brasseler, Savannah, GA, USA). Two substrates were used: hydroxyapatite and dentin. Discs were contaminated incubated in a biofilm reactor with human dental plaque. Discs were randomly assigned to an irrigant for five minutes followed by sodium thiosulfate to inactive the antimicrobial activity. DNA extraction and Next Generation Sequencing (NGS) using the MiSeq Illumina platform was completed. Number of reads, operational taxonomic units, alpha diversity, beta diversity, and relative abundance of remaining bacteria were assessed. Analysis of similarity (ANOSIM) and Kruskal-Wallis tests were performed. Results showed no statistical differences between Triton and NaOCl in antimicrobial activity; both irrigants were more antimicrobial than distilled water.