Isolation of Low Frequency Cells in Bronchoalveolar Lavage (BAL)
2012-04-18
Loading...
View/Download File
Persistent link to this item
Statistics
View StatisticsJournal Title
Journal ISSN
Volume Title
Title
Isolation of Low Frequency Cells in Bronchoalveolar Lavage (BAL)
Authors
Published Date
2012-04-18
Publisher
Type
Presentation
Abstract
The fourth leading cause of death today in the United States is lung disease which is quite often treated by lung transplantation. Lung transplant recipients seem to carry a greater risk of developing obliterative bronchiolitis (OB) which has been linked to chronic rejection of the transplanted lungs. Some recent studies have produced data that suggests the number of fibroblast and epithelial cells present in bronchoalveolar lavage (BAL) correlates to the severity of OB. Establishing a biomarker that can be used in the diagnosis of OB is important, because no clinical test exists that can be used to directly diagnose the disease. In these studies we show the populations of low frequency cells (fibroblast and epithelial) within different BAL samples. Cells are counted and sorted by type via a panning method and work has been done to prove their phenotype with immunostaining. This is an important step as no other research has documented the relative frequencies of cell populations within BAL samples, and provides a foundation to develop a clinical assay for detection of those subpopulations.
Description
Mentor: Dr. Allison Hubel
Related to
Replaces
License
Series/Report Number
Funding information
This research was supported by the Undergraduate Research Opportunities Program (UROP).
Isbn identifier
Doi identifier
Previously Published Citation
Other identifiers
Suggested citation
Albares, Luke. (2012). Isolation of Low Frequency Cells in Bronchoalveolar Lavage (BAL). Retrieved from the University Digital Conservancy, https://hdl.handle.net/11299/123068.
Content distributed via the University Digital Conservancy may be subject to additional license and use restrictions applied by the depositor. By using these files, users agree to the Terms of Use. Materials in the UDC may contain content that is disturbing and/or harmful. For more information, please see our statement on harmful content in digital repositories.