The study of nanotoxicity is essential to evaluate the safety of human
consumption and to inform the design of nanomaterials for biomedical uses. In
this work, living cells were exposed to noble metal nanoparticles with varied
size, geometry and surface chemistry in order to determine the effects these
properties have on noble metal nanoparticle uptake and toxicity. Nanoparticles
were synthesized using a variety of methods including surfactant templating,
secondary deposition, and Ostwald Ripening under controlled light conditions
in order to achieve nanomaterials with varied size and geometry. Surface
chemistry was varied by surfactant exchange using charged thiols such as 11-
mercaptoundecanoic acid and 11-mercaptoundecylamine to replace the initial
surfactants, thus yielding nanoparticles with negative or positive z-potentials.
Cellular uptake was measured using ICP-AES in exposed cells. Toxicity was
assessed by MTT and hemolysis assays. In fact, both cellular uptake of
nanoparticles and viability were dependent on the physical characteristics of
the nanomaterials varied in this study.
Additional contributors: Bryce Marquis; Christy L. Haynes (faculty mentor).
In vitro Toxicity Study of Noble Metal Nanoparticles with Varied Size, Geometry and Surface Chemistry.
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