The Chen lab developed a new technique that uses quantitative proteomics approach for site-specific ubiquitination stoichiometry analysis, named the Isotopically Balanced Quantification of Ubiquitination (IBAQ-Ub), which is a method that quantifies the absolute abundance of ubiquitination. IBAQ-Ub and in vitro ubiquitination enzymatic assays were conducted to characterize the site-specific ubiquitination dynamics and linkage specificity of RNF168 in DDR(DNA-damage-response).
This research was supported by the Undergraduate Research Opportunities Program (UROP).
Characterizing the Catalytic Activity and Specificity of RNF168 using Targeted Mass Spectrometry and Ubiquitination Stoichiometry-Based Quantitative Analysis.
Retrieved from the University of Minnesota Digital Conservancy,
Content distributed via the University of Minnesota's Digital Conservancy may be subject to additional license and use restrictions applied by the depositor.