Table detailing the design and genomic coordinates (v2 and v4) for the maize bisulfite coupled sequence capture (SeqCap-Epi-v2) probe design for profiling of the maize methylome. Released to accompany our publication on DNA methylation changes induced by tissue culture (Han et al 2017) as a supporting supplemental methods file.
Maize bisulfite coupled sequence capture (SeqCap-Epi-v2) probe design. Contained in the dataset are descriptions of the "target” regions, which are the bait regions corresponding to the sequence capture probes. Also contained are the coordinates of the target region in the B73 v4 maize genome build (Jiao et al. 2017); the strand orientation of the target; the original “target” probe capture region for the maize v2 genome build. This submission also describes the “specific” regions of interest within the captured “target” bait regions; the coordinates of the specific region in the B73 v4 maize genome build (Jiao et al. 2017); and the original “specific” region of interest for the maize v2 genome build.
National Science Foundation (IOS-1237931) to NMS
Han, Z., Crisp, P.A., Stelpflug, S., Kaeppler, S.M., Li, Q., Springer, N.M. (2018). Targeted epigenomic changes to the maize methylome resulting from tissue culture [Preprint]. bioRxiv.
Springer, Nathan M; Li, Qing; Crisp, Peter A.
(2017). Maize bisulfite coupled sequence capture (SeqCap-Epi-v2) probe design.
Retrieved from the Data Repository for the University of Minnesota,