This research introduces a method to measure changes in transit accessibility resulting from adjustments in bus-highway interactions. Operational differences between general purpose (GP) and managed lanes (ML) are measured using average travel time. Changes to transit travel time are systematically introduced to General Transit Feed Specification (GTFS) data through the use of the StopTimesEditor computer program developed for the purpose of this analysis. The methodology is tested on two express bus routes in the Minneapolis - St. Paul region (Twin Cities). The change in operating speed along portions of the selected transit routes is translated to changes in the job accessibility of the surrounding communities. The percent change in the worker-weighted average job accessibility for the area surrounding the transit routes and for the entire metropolitan region are 11.0\% and 0.26\% respectively. The methods introduced in this study can be used to evaluate the accessibility impacts of different highway operating environments for buses, or estimate the accessibility outcomes of different bus-highways scenarios.