Reprogramming is the process of converting a somatic cell into a different cell type in a controlled manner without an intermediate pluripotent state, usually by expression of exogenous transcription factors. Here, 3 transcription factors, (Pdx1, Ngn3 and MafA) incorporated in a polycistronic adenoviral vector system (Ad-PNM) was used to directly reprogram cells to beta-like cells that produce insulin in vitro. In addition, the role of innate immunity activation and open chromatin structure during reprogramming is studied to improve overall reprogramming efficiency while using the Ad-PNM adenoviral vector.
University of Minnesota M.S. thesis. December 2016. Major: Stem Cell Biology. Advisor: James Dutton. 1 computer file (PDF); vii, 45 pages.
Sripathy Rao, Srinidhi Rao.
Determination of Methods for Improved Reprogramming of Various Cell Types to Functional Beta-like Cells.
Retrieved from the University of Minnesota Digital Conservancy,
Content distributed via the University of Minnesota's Digital Conservancy may be subject to additional license and use restrictions applied by the depositor.