During my Ph.D. training, my research has been focused on two distinct topics: investigating the immunologic fuction of Virtual Memory CD8+ T cells; and elucidating the role of KLF2 in effector CD4+ T cell lineage commitment. In the first part (Chaper 2), we investigate the immunologic properties of foreign-antigen specific memory-phenotype T cells, termed Virtual Memory (VM). Our data indicates that VM cells differ functionally from "true memory" cells, yet VM cells efficiently control a bacterial (Listeria monocytogenes) infection. These data support the novel concept that naturally occurring VM cells contribute to "pre-immune" resistance to infection. In the second part (Chapter 3), we find that expression of the transcription factor Kruppel-like factor 2 (KLF2) expression varies in distinct Th subsets, and downregulation of KLF2 and the trafficking molecule S1PR1 (the well-defined target of KLF2) is required for Tfh differentiation. In addition to promoting S1PR1 expression, we also find that KLF2 induces expression of Blimp-1, which is known to oppose Tfh differentiation. Furthermore, KLF2 also promotes expression of T-bet and Gata3, and enhances Th1 differentiation. These data reveal that KLF2 plays an important role in dictating the lineage differentiation of CD4+ T cells.
University of Minnesota Ph.D. dissertation. November 2014. Major: Molecular, Cellular, Developmental Biology and Genetics. Advisor: Stephen Jameson. 1 computer file (PDF); vii, 129 pages.
Immunologic function of Virtual Memory CD8+ T cells & Role of KLF2 in effector CD4+ T cell lineage commitment.
Retrieved from the University of Minnesota Digital Conservancy,
Content distributed via the University of Minnesota's Digital Conservancy may be subject to additional license and use restrictions applied by the depositor.