Title
The effect of tenofovir on bone remodeling.
Abstract
With the introduction of Highly Active Anti-Retroviral Therapy (HAART), HIVinfected
patients have had long-term survival rates increase dramatically. A significant
sequela from HIV-infection and side effect from some HAART medications is the loss of
bone mineral density. The purpose of this study was to investigate the effects of a
common HAART medication, tenofovir, on bone cells. It was hypothesized that tenofovir
alters the gene expression of osteoblasts and osteoclasts, which creates an imbalance in
the bone remodeling system and skeletal change. To test this, mice were treated with
tenofovir, then serum was obtained to determine changes in osteocalcin, osteopontin, and
carboxyl telopeptides of type I collagen activity. Furthermore, changes in osteoclast
numbers were obtained by tartrate resistant acid phosphatase staining of mouse femurs.
While other studies have shown that gene expression is altered in tenofovir treated cells,
the present study did not find a significant difference in osteocalcin, osteopontin or
carboxyl telopeptide of type I collagen activity for tenofovir mice. However, tenofovir
decreased the number of osteoclasts in tartrate resistant acid phosphatase stained sections
in mice. Given that HIV patients taking tenofovir medication have an increase in
osteoporosis and the results of this study indicate tenofovir alone creates osteopetrotic
effect, further studies are needed to determine the relationship between HIV infection and
tenofovir.
Description
University of Minnesota M.S. thesis. August 2011. Major: Dentistry. Advisor: Dr. Kim Mansky. 1 computer file (PDF) v, 24 pages.
Suggested Citation
Dowd, Elizabeth Ann.
(2011).
The effect of tenofovir on bone remodeling..
Retrieved from the University of Minnesota Digital Conservancy,
https://hdl.handle.net/11299/116865.