Recently, a new technique was developed in Dr. Arriaga’slab to culture single muscle fibers. The cultured fibers are functional and have mitochondrial membrane potential for up to 48 hours, however the heterogeneous nature of the fibers prevent valid conclusions about ROS production and aging to be made. To be able to further analyze and provide treatment to the fibers, new technology must be developed. This study aims at creating a device based upon microfluidic theory to immobilize the fibers and thus provide a better way to analyze the fibers. In the end, we wish to fabricate a device that will test a high throughput of fibers and will allow safe treatment of drugs and quantitative measurements to be taken. To start this process, design and computer-aided simulation of the device were made.