Browsing by Author "Zimmerman, Rheanne"
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Item Effects of NOM1 on Ribosome Biogenesis(2010-04-21) Zimmerman, RheanneRibosomes translate genetic information from messenger RNA into proteins, and are therefore necessary for cell growth. Disruption of ribosome biogenesis leads to arrest in cell growth and replication, and has been identified as a precursor to some cancers. Eukaryotic ribosomes are composed of 40S and 60S subunits. The 40S subunit includes one segment of ribosomal RNA and approximately 30 proteins. The 60S subunit is composed of three segments of rRNA and approximately 50 proteins. Both are necessary to translate proteins from mRNA. NOM1 was identified by the Conklin lab because of its location at a breakpoint on chromosome 7 associated with acute myeloid leukemia. Functional studies of NOM1 have demonstrated that it: is required for cell growth and cell replication; localizes to the nucleolus; interacts with and targets several proteins to the nucleolus including Protein Phosphatase I, the oncogene MSP58 and the RNA helicase eIF4AIII; is required for production of 40S ribosomes.Item Stability of Folate and Hemoglobin in Dried Blood Spot Samples(2012-04-18) Zimmerman, RheanneDried blood spot samples provide a simple and relatively inexpensive way to collect and store blood by using specially designed filter paper, and are routinely created for infants born in the United States. Extra dried blood spot samples are often stored by state departments of health for several years, and offer a potential resource for epidemiological research. This study measured two analytes of interest, folate and hemoglobin, in dried blood spot samples from healthy Minnesota newborn subjects that were stored for 9 months at four different conditions: -80 degrees C, 4 degrees C, 25 degrees C, and a humid condition. We found that folate levels decreased significantly with warmer storage temperatures and humidity, while hemoglobin levels decreased with increased storage temperature but not humidity. The hemoglobin-folate value, used to standardize the amount of blood in the sample for measuring folate, did not decrease the standard error for our samples. Thus, for studies measuring folate using a constant size of saturated dried blood spot filter paper, measuring hemoglobin may not offer an advantage. Despite the decrease in measured values, rank order of hemoglobin, folate, and hemoglobinfolate measurements was generally preserved between storage conditions, as analyzed by general estimating equations. This indicates that dried blood spots stored under unfavorable conditions retain the ability to distinguish between differing levels of folate and hemoglobin and so may still be useful for epidemiological studies.