This readme.txt file was generated on 20211206 by Robert S. Jones ------------------- GENERAL INFORMATION ------------------- 1. Title of Dataset A Novel Methacrylate Derivative Polymer That Resists Bacterial Cell-Mediated Biodegradation Data Sharing Archive 2. Author Information Principal Investigator Contact Information Name: Robert S Jones Institution: University of Minnesota Address: 515 Delaware Street S.E.,Minneapolis, MN 55455 Email: rsjones@umn.edu ORCID: 0000-0002-9213-3657 Associate or Co-investigator Contact Information Name: Dhiraj Kumar Institution: University of Minnesota Address: 515 Delaware Street S.E.,Minneapolis, MN 55455 Email: kumard@umn.edu ORCID: 0000-0002-1008-8638 Associate or Co-investigator Contact Information Name: Debarati Ghose Institution: University of Minnesota Address: 515 Delaware Street S.E.,Minneapolis, MN 55455 Email: dghose@umn.edu ORCID: 0000-0002-4924-743X Associate or Co-investigator Contact Information Name: Isha Mutreja Institution: University of Minnesota Address: 515 Delaware Street S.E.,Minneapolis, MN 55455 Email: imutreja@umn.edu ORCID:0000-0002-8998-7563 Associate or Co-investigator Contact Information Name: Robert Bolskar Institution: TDA Research Inc Address: 12345 West 52nd Avenue, Wheat Ridge, CO 80033 Email: bolskar@tda.com ORCID: 3. Date published or finalized for release: 2021-11-22 4. Date of data collection (single date, range, approximate date) : 2020-08-17 to 2021-04-29 5. Geographic location of data collection (where was data collected?): Minneapolis, MN, USA 6. Information about funding sources that supported the collection of the data: NIH/NIDCR 1R43DE024013 7. Overview of the data (abstract): We studied biodegradation resistance of a custom synthesized (by TDA Research Inc) novel ethylene glycol ethyl methacrylate (EGEMA) with ester bond linkages that are external to the central polymer backbone when polymerized. Experiments were designed to compare degradation resistance with Ethylene glycol dimethacrylate (EGDMA) with internal ester bond linkages. The data has been published in an article titled "A Novel Methacrylate Derivative Polymer That Resists Bacterial Cell-Mediated Biodegradation" in the Journal of Biomedical Materials Research: Part B - Applied Biomaterials. -------------------------- SHARING/ACCESS INFORMATION -------------------------- 1. Licenses/restrictions placed on the data: CC0 1.0 Universal 2. Links to publications that cite or use the data: Kumar, D., Ghose, D., Bolskar, R. D., Mutreja, I., and Jones, R. S. (2021). A Novel Methacrylate Derivative Polymer That Resists Bacterial Cell-Mediated Biodegradation. Journal of Biomedical Materials Research: Part B Applied Biomaterials. Advance online publication. https://doi-org.ezp2.lib.umn.edu/10.1002/jbm.b.34972 3. Was data derived from another source? No 4. Terms of Use: Data Repository for the U of Minnesota (DRUM) By using these files, users agree to the Terms of Use. https://conservancy.umn.edu/pages/drum/policies/#terms-of-use 5. Recommended citation for the data: Kumar, Dhiraj; Ghose, Debarati; Mutreja, Isha; Bolskar, Robert; Jones, Robert S. (2021). A Novel Methacrylate Derivative Polymer That Resists Bacterial Cell-Mediated Biodegradation Data Sharing Archive. Retrieved from the Data Repository for the University of Minnesota, https://hdl.handle.net/11299/225348. --------------------- DATA & FILE OVERVIEW --------------------- 1. File List A. Filename: All Figures-Kumar-Jones-S mutans EGEMA degradation.xlsx Short description: Supplemental data that supports publication figures B. Filename: All Figures-Kumar-Jones-S mutans EGEMA degradation - csv versions.zip Short description: Supplemental data that supports publication figures in preservable format (worksheets separated and saved as csv) Figure 2_A_media activity: Esterase activity assay for THYE+Glucose media with and without S. mutans cells (in terms of p-nitrophenol (µM) synthesized from p-nitrophenol acetate) Figure 4_A_Hardness with with S: Vickers' hardness measurement for 'as prepared' discs for EGDMA and EGEMA polymers and after 9 weeks for S. mutans exposure in THYE+glucose media. On each disc 2 spots were indented for hardness. Figure 4_B_DTS with S mutans at: diametral tensile strength (MPa) measured from stress vs strain curve for 'as prepared' discs and discs' exposed to S. mutans cells in THYE+glucose media for 9 weeks. Figure 4_C_TEF with S mutans at: Total Energy to fracture (MJ/m^3) was measured from stress vs strain curve by calculating area under the curve for 'as prepared' discs and discs' exposed to S. mutans cells in THYE+glucose media for 9 weeks. Figure 4_D_yielding point with: Yielding point (MPa) measurement was performed by drawing a tangent between 0.5-1.0% strain on DTS vs strain curve and marking the point where the shpae of curve strated to change. Figure 5_A_Water CA with S muta: Captive bubble water contact angle (°) was measured by immersing the 'as prepared' and discs exposed to S. mutans in water and injecting an air bubble by software controlled system and capturing the image, followed by processing the image using ImageJ. Figure 5_B_Water sorption with: Water sorption (wt%) was calculated using the weight of washed discs and weight of discs after vacuum drying. Figure 5_C_OCT Minor defects wi: Minor defect (%) was also calculated using the Optical Coherence Tomography (OCT) images, processed using a MATLAB script, with threshold scattering intensity 220dB at material-void interface. Figure S1_Kaplan Meier Survival: (8 Samples in each set) the weight for each disc was measured at the end of each week and finally when relative weight loss reached 10% (assumed threshold), the discs was assumed structurally unstable (for EGDMA at week 9 only 1 discs was ok and for EGEMA three discs were ok). 2. Relationship between files: The zipped files are duplicates of the Excel file, transformed for long-term preservation. -------------------------- METHODOLOGICAL INFORMATION -------------------------- 1. Description of methods used for collection/generation of data: See publication link: http://doi.org/10.1002/jbm.b.34972 for all details. We studied biodegradation resistance of a custom synthesized (by TDA Research Inc) novel ethylene glycol ethyl methacrylate (EGEMA) with ester bond linkages that are external to the central polymer backbone when polymerized. Experiments were designed to compare degradation resistance with Ethylene glycol dimethacrylate (EGDMA) with internal ester bond linkages. The data has been published in an article titled "A Novel Methacrylate Derivative Polymer That Resists Bacterial Cell-Mediated Biodegradation" in the Journal of Biomedical Materials Research: Part B - Applied Biomaterials. The data in this record supports the figures published in the manuscript. Figure 2_A_media activity: Esterase activity assay for THYE+Glucose media with and without S. mutans cells (in terms of p-nitrophenol (µM) synthesized from p-nitrophenol acetate) Figure 4_A_Hardness with with S: Vickers' hardness measurement for 'as prepared' discs for EGDMA and EGEMA polymers and after 9 weeks for S. mutans exposure in THYE+glucose media. On each disc 2 spots were indented for hardness. Figure 4_B_DTS with S mutans at: diametral tensile strength (MPa) measured from stress vs strain curve for 'as prepared' discs and discs' exposed to S. mutans cells in THYE+glucose media for 9 weeks. Figure 4_C_TEF with S mutans at: Total Energy to fracture (MJ/m^3) was measured from stress vs strain curve by calculating area under the curve for 'as prepared' discs and discs' exposed to S. mutans cells in THYE+glucose media for 9 weeks. Figure 4_D_yielding point with: Yielding point (MPa) measurement was performed by drawing a tangent between 0.5-1.0% strain on DTS vs strain curve and marking the point where the shpae of curve strated to change. Figure 5_A_Water CA with S muta: Captive bubble water contact angle (°) was measured by immersing the 'as prepared' and discs exposed to S. mutans in water and injecting an air bubble by software controlled system and capturing the image, followed by processing the image using ImageJ. Figure 5_B_Water sorption with: Water sorption (wt%) was calculated using the weight of washed discs and weight of discs after vacuum drying. Figure 5_C_OCT Minor defects wi: Minor defect (%) was also calculated using the Optical Coherence Tomography (OCT) images, processed using a MATLAB script, with threshold scattering intensity 220dB at material-void interface. Figure S1_Kaplan Meier Survival: (8 Samples in each set) the weight for each disc was measured at the end of each week and finally when relative weight loss reached 10% (assumed threshold), the discs was assumed structurally unstable (for EGDMA at week 9 only 1 discs was ok and for EGEMA three discs were ok). 2. Instrument- or software-specific information needed to interpret the data: Matlab R2019a, GraphPad Prism v8.0.2 3. People involved with sample collection, processing, analysis and/or submission: sample collection, processing: D. Kumar, D. Ghose, B. Bolskar, and I. Mutreja. Analysis: D. Kumar, D Ghose, B Bolskar, I Mutreja, and R. Jones