------------------- GENERAL INFORMATION ------------------- 1. Title of Dataset Data for "Microfluidic long DNA sample preparation from cells" published as Lab Chip, 2019, 19, 281 2. Author Information Principal Investigator Contact Information Name: Paridhi Agrawal Institution: University of Minnesota Email: agraw135@umn.edu Associate or Co-investigator Contact Information Name: Kevin Dorfman Institution: University of Minnesota 3. Date of data collection: 2018-09-01 to 2018-10-30 4. Information about funding sources that supported the collection of the data: Sponsorship: NIH (R21-HG009208); NNCI under Award Number ECCS-1542202 -------------------------- SHARING/ACCESS INFORMATION -------------------------- 1. Licenses/restrictions placed on the data: CC0 1.0 Universal 2. Links to publications that cite or use the data: Agrawal, P., & Dorfman, K. D. (2019). Microfluidic long DNA sample preparation from cells. Lab on a Chip, 19(2), 281–290. http://doi.org/10.1039/C8LC01163J ----------------------------------------- DATA-SPECIFIC INFORMATION FOR: Fig5_Annotate.tif ----------------------------------------- 1. Description: Fig5_Annotate.tif is the tiff file of Fig. 5 that has all the molecules numbered for analysis shown in MWD.csv ----------------------------------------- DATA-SPECIFIC INFORMATION FOR: MWD.csv ----------------------------------------- 1. Description: MWD.csv has the molecular weight distribution of the 122 DNA molecules in Fig. 5 of the manuscript, that is represented as a histogram in Fig. 6. Columns A to I are raw data from ImageJ. Column J is calibrated length measured in microns based on conversion factor of 1 µm = 15.86 pixels. The MW (kbp) in column K is the molecular weight of the molecules in kilo base pair unit calculated from the length measured in microns in ImageJ using the conversion factor of 1 µm = 3.13 kbp. 2. Number of variables: 10 3. Number of rows: 123 rows 4. Missing data codes: N/A 5. Variable List A. Name: Area Description: Area of the selected line in micrometer squared. B. Name: X Description: X centroid of the line selection: average of the x coordinates of all pixels in the line selection. C. Name: Y Description: Y centroid of the line selection: average of the y coordinates of all pixels in the line selection. D. Name: XM Description: X center of mass: Brightness-weighted average of the x coordinates of all the pixels in the selection. E. Name: YM Description: Y center of mass: Brightness-weighted average of the y coordinates of all the pixels in the selection. F. Name: Angle Description: Angle between the line selection and a line parallel to the x axis of the image. G. Name: IntDen Description: Integrated density: The product of area and average gray value of the line selection. H. Name: RawIntDen Description: Raw Integrated Density: sum of the values of the pixels in the line selection. I. Name: Length Description: Length of the line selection in micrometer. J. Name: MW (kbp) Description: Molecular weight of the molecule in kilo base pair units corresponding to the line selection. ----------------------------------------- DATA-SPECIFIC INFORMATION FOR: QubitData.xlsx ----------------------------------------- 1. Description: QubitData.xlsx shows the DNA and protein concentration data of our device samples that is presented in the manuscript. This file was exported from the Qubit Fluorometer 2.0. The result of interest is the Original sample concentration displayed in Column G. For both DNA and protein, emission spectra is green and only the Green RFU is measured. Far red RFU is the standard readout when other nucleic acids are measured, and is not read during the protein and double-stranded DNA assay. 2. Number of variables: 17 3. Number of cases/rows: 28 rows 4. Missing data codes: Code/symbol Definition Code/symbol Definition 5. Variable List A. Name: Run ID Description: This represents date of experiment and a unique serial number generated by the Fluorometer for the sample. B. Name: Assay Name Description: This shows the assay performed, either protein or dsDNA Broad range. C. Name: Test Name Description: This is the default sample name given to the experiment by the instrument. D. Name: Test Date Description: This shows the date and time of the measurement. E. Name: Qubit® tube conc. Description: This shows the concentration of protein or nucleic acid, depending on the assay performed, in the tube which has our sample diluted in the kit-provided buffer. F. Name: Units Description: This shows the units in which Qubit tube concentration in Column E is measured. µg/mL G. Name: Original sample conc. Description: This column calculates the actual concentration of our concentrated sample. H. Name: Units Description: This shows the units in which Original sample concentration in Column G is measured. ng/µL I. Name: Sample Volume (µL) Description: This shows the volume of our sample mixed with buffer to make a total volume of 200 µL in the assay tube. J. Name: Dilution Factor Description: This is the dilution factor of our sample in the assay tube. 200/value in Column I. K. Name: Std 1 RFU Description: This shows the intensity recorded for the Standard Solution 1 provided with the kit corresponding to the assay being performed. This is used to calibrate and convert the sample fluorescent intensity measured to concentration by the instrument. L. Name: Std 2 RFU Description: This shows the intensity recorded for the Standard Solution 2 provided with the kit corresponding to the assay being performed. This is used to calibrate and convert the sample fluorescent intensity measured to concentration by the instrument. M. Name: Std 3 RFU Description: This shows the intensity recorded for the Standard Solution 3 provided with the kit corresponding to the assay being performed. This is used to calibrate and convert the sample fluorescent intensity measured to concentration by the instrument. (DNA assay has two standard solutions whereas protein assay has three standard solutions). N. Name: Excitation Description: This shows the laser color used for sample excitation. O. Name: Emission Description: This shows the laser color detected during sample emission. P. Name: Green RFU Description: This shows the intensity recorded from the sample being assayed for. Q. Name: Far Red RFU Description: This is not applicable for the assays being performed and is therefore empty.