This readme.txt file was generated on 2019-12-24 by Anh K. Tran ------------------- GENERAL INFORMATION ------------------- 1. Title of Dataset Morphometric Measurements of Field and Laboratory-Reared Spotted-Wing Drosophila (2017-2018) 2. Author Information Principal Investigator Contact Information Name: Anh K. Tran Institution: University of Minnesota Address: 1980 Fowell Ave 219 Hodson Hall St. Paul, MN 55108 Email: aktran@umn.edu Associate or Co-investigator Contact Information Name: W. D. Hutchison Institution: University of Minnesota Address: 1980 Fowell Ave 219 Hodson Hall St. Paul, MN 55108 Email: hutch002@umn.edu Associate or Co-investigator Contact Information Name: Mark K. Asplen Institution: Metropolitan State University Address: 700 East Seventh Street Saint Paul, MN 55106 Email: mark.asplen@metrostate.edu 3. Date of data collection: 2017-06-17 to 2018-11-07 4. Geographic location of data collection (where was data collected?): University of Minnesota Outreach, Research and Education (UMORE) Park Rosemount, Minnesota 5. Information about funding sources that supported the collection of the data: Sponsorship: Minnesota Invasive Terrestrial Plants and Pests Center (through the the Environment and Natural Resources Trust Fund; mitppc.umn.edu) -------------------------- SHARING/ACCESS INFORMATION -------------------------- 1. Licenses/restrictions placed on the data: Attribution-NonCommercial 3.0 United States 2. Links to publications that cite or use the data: Tran AK, Hutchison WD, Asplen MK (2020) Morphometric criteria to differentiate Drosophila suzukii (Diptera: Drosophilidae) seasonal morphs. PLoS ONE 15(2): e0228780. https://doi.org/10.1371/journal.pone.0228780 3. Links to other publicly accessible locations of the data: 4. Links/relationships to ancillary data sets: 5. Was data derived from another source? If yes, list source(s): 6. Recommended citation for the data: Tran, Anh K.; Hutchison, W. D.; Asplen, Mark K.. (2019). Morphometric Measurements of Field and Laboratory-Reared Spotted-Wing Drosophila (2017-2018). Retrieved from the Data Repository for the University of Minnesota, http://hdl.handle.net/11299/209895. --------------------- DATA & FILE OVERVIEW --------------------- 1. File List A. Filename: data.xlsx Short description: Contains measurement data for summer and winter morphs of Drosophila suzukii B. Filename: Readme.txt Short description: Describes the dataset 2. Relationship between files: 3. Additional related data collected that was not included in the current data package: 4. Are there multiple versions of the dataset? yes/no No -------------------------- METHODOLOGICAL INFORMATION -------------------------- 1. Description of methods used for collection/generation of data: For more information refer to: https://doi.org/10.1371/journal.pone.0228780 Lab flies: Drosophila suzukii colony was established in the laboratory by collecting infested raspberry fruit in 2016, produced at the University of Minnesota Outreach, Research and Education (UMORE) Park in Rosemount, MN. Protocols and methods for rearing D. suzukii summer and winter morphs were performed as described by Stephens et al. 2016 (Stephens AR, Asplen MK, Hutchison WD, Venette RC. Cold hardiness of winter-acclimated Drosophila suzukii (Diptera: Drosophilidae) adults. Environ Entomol. 2015;44(6):1619–26. pmid:26317777) Summer morphs were reared in narrow polystyrene vials with foam plugs. Each vial contained approximately 5 milliliter of an agar-yeast-cornmeal diet and a strip of filter paper to reduce mortality from condensation. Vials were stored at 22 ± 1°C, a photoperiod of 16:8 (light:dark) hours and relative humidity of 60 ± 10%. Adult winter morph D. suzukii were produced by placing vials with 1 to 3-day-old eggs from summer morphs and into a growth chamber set at 10 ± 1°C, a photoperiod of 12:12 (light:dark) hours and relative humidity of 70 ± 10%. At 10°C, winter morphs typically eclose within approximately 56 days. Adult summer and winter morph flies that were approximately 2-days-old (from eclosion) were used for the study. Field flies: Adult D. suzukii were collected throughout the 2017 and 2018 growing seasons, in the metropolitan area of Minneapolis-St. Paul, MN. In both years at each site, two commercial trapping systems were used: Pherocon® SWD and Scentry. Pherocon® SWD traps consisted of a Pherocon® SWD lure and apple cider vinegar with a drop of liquid soap to lower surface tension. The Scentry trap consisted of a Scentry SWD lure and water with a drop of liquid soap, to lower surface tension. At each participating location, traps were monitored weekly to remove the contents, and transfer samples to the laboratory for processing, identification, and measurement. In 2017, traps were deployed from 15 May to 31 August except at UMORE Park where traps were taken down on 5 December. In 2018, traps were deployed from 14 May to 25 September except at UMORE Park where traps were taken down on 11 November. Lures were replaced approximately once per month and liquids (apple cider vinegar or water) were replaced weekly. As temperatures approached 0°C, the apple cider vinegar and water were replaced with propylene glycol. Measurements: Individual flies from laboratory colonies or field sites were initially placed under a stereo microscope with 8x – 35x magnification. The wings and hind legs were removed from the thorax by dissecting individuals on a microscope slide with phosphate-buffered saline (DPBS), 1X with calcium and magnesium solution. Dissected contents were left on the microscope slide. Wing length, wing width and hind tibia length were measured using a compound light microscope with 4x – 100x magnification and a calibrated reticle. Measurements were only recorded from one wing and leg per fly. Wing length was measured from the base of the wing to the apex of the R4 + 5 vein. Wing width was measured from the costal margin to the posterior edge following the medial cubital cross vein. The longest dimension of the hind tibia was measured. The sex of each individual was recorded. 2. Methods for processing the data: For more information refer to: https://doi.org/10.1371/journal.pone.0228780 3. Instrument- or software-specific information needed to interpret the data: 4. Standards and calibration information, if appropriate: 5. Environmental/experimental conditions: 6. Describe any quality-assurance procedures performed on the data: 7. People involved with sample collection, processing, analysis and/or submission: ----------------------------------------- DATA-SPECIFIC INFORMATION FOR: data.xlsx ----------------------------------------- 1. Number of variables: 7 2. Number of cases/rows: 429 3. Missing data codes: no missing data 4. Variable List A. Name: Date Description: For lab specimens, the date that the specimen was dissected For field specimens, the date that the speciman was collected in the field B. Name: Morph Description: Morphs were known for lab specimens. W = winter morph S = summer morph F = field-caught specimen, morph unknown C. Name: Type Description: lab = specimen raised in lab field = specimen caught in the field D. Name: Sex Description: M = Male F = Female E. Name: Wing length Description: Measured from the base of the wing to the apex of the R4 + 5 vein (in mm) F. Name: Wing width Description: Measured from the costal margin to the posterior edge following the medial cubital cross vein (in mm) G. Name: Hind tibia length Description: Longest dimension of the hind tibia (in mm)