------------------- GENERAL INFORMATION ------------------- 1. Title: R Code and Output Supporting: Oviposition substrate preferences of two endangered prairie butterflies in an ex situ conservation environment 2. Author information: Name: Amaya Thomas Affiliation: Conservation Department, Minnesota Zoo, Apple Valley, MN, USA Email: amaya.thomas@state.mn.us ORCID: 0009-0002-7390-1195 Name: John Fieberg Affiliation: Department of Fisheries, Wildlife and Conservation Biology, University of Minnesota, St. Paul, Minneapolis, MN, USA Email: jfieberg@umn.edu ORCID: 0000-0002-3180-7021 Name: Erik Runquist Affiliation: Conservation Department, Minnesota Zoo, Apple Valley, MN, USA Email: erik.runquist@state.mn.us ORCID: 0009-0009-0826-5154 Name: Cale Nordmeyer Affiliation: Conservation Department, Minnesota Zoo, Apple Valley, MN, USA Email: cale.nordmeyer@state.mn.us ORCID: 0000-0002-8826-251X Name: Seth Stapleton Affiliation: Conservation Department, Minnesota Zoo, Apple Valley, MN, USA; Department of Fisheries, Wildlife and Conservation Biology, University of Minnesota, St. Paul, Minneapolis, MN, USA Email: seth.stapleton@state.mn.us ORCID: 0009-0001-6422-6880 3. Description: This repository contains the data, supplementary material, and R code and associated output supporting the results reported in: Thomas, A., Fieberg, J., Runquist, E., Nordmeyer, C., & Stapleton, S. Oviposition substrate preferences of two endangered prairie butterflies in an ex situ conservation environment. -------------------------- Version History -------------------------- -------------------------- SHARING/ACCESS INFORMATION -------------------------- --------------------- FILE OVERVIEW --------------------- 1. Oviposition_Data_2023.csv = Dakota skipper and Poweshiek skipperling egg data 2. Oviposition_SA_2023.csv = Surface area data 3. Final_Oviposition_Analysis.Rmd and Final_Oviposition_Analysis.html = R code (and associated html file containing output from running the code) for conducting the analyses described in Thomas et al. (In Review) 4. Figure_S1.tif = Supplementary figure 1 for Thomas et al. Figure includes images of the experimental cage setup and images of a female Dakota skipper and Poweshiek skipperling. 5. Figure_S2.tif = Supplementary figure 2 for Thomas et al. Figure shows bootstrap distributions of the individual selection ratios. 6. Figure_S3.tif = Supplementary figure 3 for Thomas et al. Figure shows bootstrap distributions of the population-level selection ratios. 7. Figure_S4.tif = Supplementary figure 4 for Thomas et al. Figure shows the individual selection ratios and their 95% CI's. -------------------------- METHODOLOGICAL INFORMATION -------------------------- 1. Methods: Between 20 June and 26 July 2023, we selected ten gravid Dakota skipper females and 13 gravid Poweshiek skipperling females and offered them three graminoid species simultaneously: prairie dropseed, tussock sedge, and smooth brome. We individually placed females of each butterfly into a 1ft. cubic mesh pop-up cage containing a 4in. pot filled with soil. The soil held four 3in. water tubes in place, with three of the tubes containing fresh cuttings of the three graminoids and the fourth tube containing flower cuttings. We collected eggs from the females at the end of each day, starting the day the female mated and continuing until she died, and we recorded the substrate on which all eggs were laid. For complete methodological details, please refer to Thomas et al. (In Review). 2. Instrument- or software-specific information needed to interpret the data: Programs were written for Program R (R Core Team 2024) and full session information is provided at the bottom of the html file. Packages used are provided at the top of the html file. ----------------------------------------- DATA-SPECIFIC INFORMATION FOR: Oviposition_Data_2023.csv ----------------------------------------- Number of variables: 5 Number of cases/rows: 467 Variable List A. Spp = species, either HEDA (Dakota skipper) or OAPO (Poweshiek skipperling) B. ID = individual's unique ID C. Date = date the eggs were collected (MM/DD/YYYY) D. Eggs = number of eggs collected E. Substrate = substrate on which the eggs were collected, including PDS (prairie dropseed), TS (tussock sedge), SMBR (smooth brome), FLOWER, CAGE, POT, SOIL, TUBE (water tube), and VIAL (one female Dakota skipper laid an egg in a vial when collecting her eggs -- we excluded this data point from our analysis). ----------------------------------------- DATA-SPECIFIC INFORMATION FOR: Oviposition_SA_2023.csv ----------------------------------------- Number of variables: 2 Number of cases/rows: 8 Variable List A. Substrate = substrates include PDS (prairie dropseed), TS (tussock sedge), SMBR (smooth brome), FLOWER, CAGE, POT, SOIL, TUBE (water tube). B. SA = surface area in mm^2 ----------------------------------------- FUNDING ----------------------------------------- Funding for this project was provided by the Great Lakes Restoration Initiative through the U.S. Fish and Wildlife Service's Threatened and Endangered Species Template, as well as the Environment and Natural Resources Trust Fund as recommended by the Legislative-Citizen Commission on Minnesota Resources. Supplemental funding was provided by the Minnesota Zoo and Minnesota Zoo Foundation. All butterflies utilized in these experiments were reared and maintained following established husbandry protocols developed by the Minnesota Zoo's Pollinator Conservation Initiative. Activities were authorized under the U.S. Fish and Wildlife Service Native Endangered and Threatened Species Recovery Permit ES64079B and the State of Minnesota Special Permit 32847. ----------------------------------------- REFERENCES ----------------------------------------- R Core Team. (2024). R: A Language and Environment for Statistical Computing. R Foundation for Statistical Computing, Vienna, Austria. https://www.r-project.org/