This readme.txt file was generated on <2020-08-07> by ------------------- GENERAL INFORMATION ------------------- 1. Title of Dataset Supporting data for "Sulfide impacts population oscillations of wild rice" 2. Author Information Principal Investigator Contact Information Name:Nathan W. Johnson Institution: University of MN Duluth Address: Email:nwjohnso@d.umn.edu ORCID: Associate or Co-investigator Contact Information Name:Sophia LaFond-Hudson Institution:University of MN Duluth Address: Email:lafo0062@d.umn.edu ORCID: Associate or Co-investigator Contact Information Name:John Pastor Institution:University of MN Duluth Address: Email:jpastor@d.umn.edu ORCID: Associate or Co-investigator Contact Information Name:Brad Dewey Institution:University of MN Duluth Address: Email:bdewey@d.umn.edu ORCID: 3. Date of data collection (single date, range, approximate date) 2014-09-01 to 2019-10-01 4. Geographic location of data collection (where was data collected?): UMD Research Farm 5. Information about funding sources that supported the collection of the data: MN SeaGrant, Fond du Lac Lake Superior Band of Chippewa -------------------------- SHARING/ACCESS INFORMATION -------------------------- 1. Licenses/restrictions placed on the data: CC0 1.0 Universal (http://creativecommons.org/publicdomain/zero/1.0/) 2. Links to publications that cite or use the data: none yet 3. Links to other publicly accessible locations of the data: none 4. Links/relationships to ancillary data sets: none 5. Was data derived from another source? If yes, list source(s): no 6. Recommended citation for the data: LaFond-Hudson, Sophia; Johnson, Nathan W; Pastor, John; Dewey, Brad. (2020). Supporting data for "Sulfur geochemistry impacts population oscillations of wild rice (Zizania palustris)". Retrieved from the Data Repository for the University of Minnesota, https://doi.org/10.13020/cq0g-r486. --------------------- DATA & FILE OVERVIEW --------------------- 1. File List: sulfur iron litter wild rice mesocosm data.xlsx Short description: Contains the mesocosm population and geochemical data, in two different worksheets, described below. A. Worksheet: Geochemical data Short description: Porewater data was collected in 2019 using passive diffusion samplers (peepers). The porewater was sampled at 4 depths for sulfide, iron, and pH. B. Worksheet: Population data Short description: Data on population traits were collected annually 2014-2019. 40 populations total were exposed to one of 8 treatment combinations of sulfate, iron, and litter, resulting in five replicates of each treatment. 2. Relationship between worksheets: The geochemical porewater data was sampled from sediment of each of the 40 mesocosm populations sampled in the population dataset. 3. Additional related data collected that was not included in the current data package: 4. Are there multiple versions of the dataset? yes/no no If yes, list versions: Name of file that was updated: i. Why was the file updated? ii. When was the file updated? Name of file that was updated: i. Why was the file updated? ii. When was the file updated? -------------------------- METHODOLOGICAL INFORMATION -------------------------- 1. Description of methods used for collection/generation of data: Geochemical data: Passive diffusion samplers were filled with deoxygenated, MilliQ water and placed in sediment for two weeks to equilibrate. After two weeks, samplers were removed and placed in a glove bag filled with nitrogen to extract porewater from 4 depths. Porewater was immediately sampled for pH using a pH probe, and absorbance of iron and sulfide were measured using a field spectrophotometer. Population data: All traits involved either counting individual plants or seeds, or harvesting a subset of six plants from each population to weigh for vegetative biomass, seed mass, etc. Plant tissues were dried before weighing. 2. Methods for processing the data: Absorbance was converted to concentration using the slope of a calibration curve. The ion activity product (IAP) was calculated from the concentration of sulfide, iron, and pH. Population traits involving mass were measured on a subset of 6 plants and extrapolated to the full size of the population. 3. Instrument- or software-specific information needed to interpret the data: 4. Standards and calibration information, if appropriate: 5. Environmental/experimental conditions: Populations were exposed to 1 of 8 different experimental conditions resulting from the combination of sulfate, iron, and litter addition. For populations receiving sulfate addition, the surface water was maintained at 300 ppm sulfate by adding appropriate amounts of sodium sulfate. For populations receiving iron addition, iron was tripled relative to the background total iron concentration in sediment. Iron (II) chloride was added directly to sediment via standpipes and a porous ring buried in the sediment. For populations receiving litter addition, total aboveground biomass was removed, dried, and returned to the mesocosms. In treatments not receiving litter, aboveground biomass was not returned. 6. Describe any quality-assurance procedures performed on the data: 7. People involved with sample collection, processing, analysis and/or submission: The authors listed collected, processed, and analyzed the data. ----------------------------------------- DATA-SPECIFIC INFORMATION FOR: [Geoechemical data] ----------------------------------------- 1. Number of variables: 15 2. Number of cases/rows: 249 3. Missing data codes: Code/symbol NA Definition Sample was not able to be measured or value was unable to be calculated because it required a sample that was not collected. Code/symbol Definition 4. Variable List A. Name: Tank Description: A unique id number given to each of the 40 mesocosms used in the experiment. B. Name: depth Description: The depth (in cm) of the porewater sample below the sediment-water interface. Negative values indicate samples in sediment, positive values indicate samples taken from the surface water (ie. 2 cm above sediment). C. Name: Trt Description: A number assigned to each of the 8 geochemical treatments tested in the experiment. 1: Addition of sulfate, iron, and litter 2: Addition of iron and litter 3: Addition of sulfate and iron 4: Addition of iron 5: Addition of sulfate and litter 6: Addition of litter 7: Addition of sulfate 8: No addition D. Name: Fe Description: 0: Mesocsom did not receive iron addition 1: Mesocosm received iron addition E. Name: L Description: 0: Mesocsom did not receive litter addition (instead removed) 1: Mesocosm received litter addition F. Name: S Description: 0: Mesocsom did not receive sulfate addition 1: Mesocosm received sulfate addition G. Name: month Description: The month porewater was sampled. july: July sept: September H. Name: fe2 Description: The concentration of Fe2+ measured in porewater in micromoles per liter. I. Name: sulfide Description: The concentration of sulfide measured in porewater in micromoles per liter. J. Name: ph Description: The pH of porewater. K. Name: H+ Description: The concentration of hydrogen ions in porewater (mol/L), calculated from ph (H+ =10^-ph) L. Name: bisulfide Description: The concentration of bisulfide ions in porewater (mol/L), calculated from measured sulfide and ph. bisulfide = Ka*sulfide/(H+ + Ka) M. Name:IAP Description: Ion activity product = bisulfide (mol/L)* fe2 (mol/L) / H+ N. Name: Fe2+ Description: The concentration of Fe2+ measured in porewater in moles per liter. O. Name: HS.H Description: The concentration of bisulfide divided by the concentration of hydrogen ions in mol/L ----------------------------------------- DATA-SPECIFIC INFORMATION FOR: [Population data] ----------------------------------------- 1. Number of variables:16 2. Number of cases/rows: 241 3. Missing data codes: Code/symbol NA Definition Variable not sampled. 4. Variable List A. Name: Tank Description: A unique id number given to each of the 40 mesocosms used in the experiment. B. Name: Year Description: The year the variable was measured C. Name: Trt Description: A number assigned to each of the 8 geochemical treatments tested in the experiment. 1: Addition of sulfate, iron, and litter 2: Addition of iron and litter 3: Addition of sulfate and iron 4: Addition of iron 5: Addition of sulfate and litter 6: Addition of litter 7: Addition of sulfate 8: No addition D. Name: Fe Description: 0: Mesocsom did not receive iron addition 1: Mesocosm received iron addition E. Name: L Description: 0: Mesocsom did not receive litter addition (instead removed) 1: Mesocosm received litter addition F. Name: S Description: 0: Mesocsom did not receive sulfate addition 1: Mesocosm received sulfate addition G. Name: germination Description: The number of plants that germinated, including seedlings that remained rooted, seedlings that released from the sediment (floaters), and seedlings that were thinned. H. Name: potential_survivors Description: The number of plants that survived to the end of the year plus the number of seedlings that were thinned. I. Name: filled_seed_ratio Description:The proportion of seeds that were filled to the total number of peduncles. J. Name: mass_per_seed Description: The average mass in mg of individual filled seeds. Calculated from total seed mass/number of seeds. K. Name: pop_seedmass Description:The total mass in g of all filled seeds produced by a population L. Name: seeds_per_plant Description: The average number of seeds produced per plant in a population M. Name: biomass_density Description: The mass of aboveground vegetation per square meter (g/m2) N. Name: pop_biomass Description: The total mass of a population (g) O. Name: plant_count Description: The total number of plants remaining at the end of the growing season. P: Name: avg_plant_wt Description: The average weight of individual plants in each population. Calculated from population biomass / plant count