This codebook.txt file was generated on 20171204 by Janet M Dubinsky ------------------- GENERAL INFORMATION ------------------- 1. Title of Dataset Abnormal Recovery After Stress in Huntingtons Disease Mice 2. Author Information Principal Investigator Contact Information Name: Janet M Dubinsky Institution: University of Minnesota Address: 6-145 Jackson Hall 321 Church St. SE Minneapolis, MN 55455 Email: dubin001@umn.edu Associate or Co-investigator Contact Information Name: William Engeland Institution: University of Minnesota Address: 6-145 Jackson Hall 321 Church St. SE Minneapolis, MN 55455 Email: engel001@umn.edu Associate or Co-investigator Contact Information Name: Arif Hamid Institution: Address: Email: Associate or Co-investigator Contact Information Name: Lori Zacharoff Institution: Address: Email: 3. Date of data collection (single date, range, approximate date) 20070327 - 20110328 4. Geographic location of data collection (where was data collected?): Minneapolis, MN USA 5. Information about funding sources that supported the collection of the data: This work was funded by CHDI, Inc and the Strom Family Fund to JMD. -------------------------- SHARING/ACCESS INFORMATION -------------------------- 1. Licenses/restrictions placed on the data: Attribution-NonCommercial-NoDerivs 3.0 United States 2. Links to publications that cite or use the data: Submitted; this information to be updated. 3. Links to other publicly accessible locations of the data: N/A 4. Links/relationships to ancillary data sets: N/A 5. Was data derived from another source? No. 6. Recommended citation for the data: Zacharoff, Lori; Hamid, Arif; Engeland, William C; Dubinsky, Janet M. (2018). Abnormal Recovery from Acute Stress in Huntingtons Disease Mice. Retrieved from the Data Repository for the University of Minnesota, http://hdl.handle.net/11299/194497. --------------------- DATA & FILE OVERVIEW --------------------- 1. File List A. Filename: Fig1Data.xls Short description: Morning and evening corticosterone levels in R6/2 mice and control mice by sex. B. Filename: Fig2Data.xls Short description: Corticosterone levels in R6/2 mice and control mice before and immediately after restraint stress and an hour after recovery from restraint stress. C. Filename: Fig3Data.xls Short description: Corticosterone levels in Q140 or BACHD mice and control mice before and immediately after restraint stress and an hour after recovery from restraint stress. D. Filename: Fig4Data.xls Short description: Corticosterone levels in response to dexamethasone suppression and ACTH stimulation in R6/2 mice and littermate controls. E. Filename: Fig5Data.xls Short description: Adrenal and serum corticosterone levels from the same R6/2 (red) and littermate control (black) mice. F. Filename: Fig7Data.xls Short description: GR immunofluorescence, total intensity/total area, for R6/2 and littermate control mice. 2. Relationship between files: Sequential experiments 3. Additional related data collected that was not included in the current data package: N/A 4. Are there multiple versions of the dataset? No. -------------------------- METHODOLOGICAL INFORMATION -------------------------- See paper. 1. Description of methods used for collection/generation of data: We analyzed serum corticosterone levels from R6/2, Q140 and BACHD mice either diurnally (R6/2) or after restraint stress and subsequent recovery (all 3 models). Glucocorticoid secretion was also examined (R6/2) following dexamethasone suppression and ACTH stimulation. Blood samples were collected either by submandibular puncture or as trunk blood at sacrifice. Plasma corticosterone levels were assayed by RIA. Regional brain glucocorticoid receptor (GR) levels were visualized using immunofluorescence. 2. Methods for processing the data: N/A 3. Instrument- or software-specific information needed to interpret the data: N/A 4. Standards and calibration information, if appropriate: N/A 5. Environmental/experimental conditions: N/A 6. Describe any quality-assurance procedures performed on the data: N/A 7. People involved with sample collection, processing, analysis and/or submission: Zacharoff collected and analyzed the majority of the data. Hamid collected and analyzed data in 2 figures. ----------------------------------------- DATA-SPECIFIC INFORMATION FOR: Table1Data.xls ----------------------------------------- 1. Number of variables: 8 2. Number of rows: 44 3. Missing data codes: N/A 4. Variable List A. Name: animal # Description: animal identification number B. Name: age (wk) Description: age in weeks C. Name: genotype Description: genotype of mouse (R6/2 or littermate control, lm cntl) D. Name: sex Description: F=female, M=male E. Name: restraint condition Description: treatment of animal (restrained or none) before scarifice F. Name: Adrenals mg/g body weight Description: ratio of adrenal weight to body weight G. Name: Adrenals (mg) Description: wet weight of adrenals at harvest H. Name: body weight (g) Description: body weight immediately prior to sacrifice ----------------------------------------- DATA-SPECIFIC INFORMATION FOR: Fig1Data.xls ----------------------------------------- 1. Number of variables: 16 2. Number of rows: 64 3. Missing data codes: N/A 4. Variable List A. Name: A<13 wk Description: age less than 13 weeks B. Name: genotype Description: genotype of mouse C. Name: sex Description: F=female, M=male D. Name: age Description: age in weeks E. Name: animal # Description: animal identification number F. Name: lm cntl Description: littermate mouse G. Name: WT Description: wild type mouse H. Name: R6/2 Description: R6/2 transgenic mouse I. Name: M Description: male J. Name: F Description: female K. Name: [cort] ng/ml Description: concentration of corticosterone in ng/ml L. Name: mean Description: mean M. Name: SD Description: standard deviation N. Name: N Description: number of measurements O. Name: am Description: morning P. Name: pm Description: evening ----------------------------------------- DATA-SPECIFIC INFORMATION FOR: Fig2Data.xls ----------------------------------------- 1. Number of variables: 15 2. Number of rows: 51 3. Missing data codes: N/A 4. Variable List A. Name: time (min) Description: time in min relative to onset of 30 min of restraint stress B. Name: age (wk) Description: age in weeks C. Name: genotype Description: genotype of mouse D. Name: sex Description: F=female, M=male E. Name: age Description: age in weeks F. Name: animal # Description: animal identification number G. Name: lm cntl Description: littermate mouse H. Name: WT Description: wild type mouse I. Name: R6/2 Description: R6/2 transgenic mouse J. Name: M Description: male K. Name: F Description: female L. Name: [cort] ng/ml Description: concentration of corticosterone in ng/ml M. Name: mean Description: mean N. Name: SD Description: standard deviation O. Name: N Description: number of measurements ----------------------------------------- DATA-SPECIFIC INFORMATION FOR: Fig3Data.xls ----------------------------------------- 1. Number of variables: 14 2. Number of rows: 74 3. Missing data codes: N/A 4. Variable List A. Name: time (min) Description: time in min relative to onset of 30 min of restraint stress B. Name: age (wk) Description: age in weeks C. Name: genotype Description: genotype of mouse D. Name: sex Description: F=female, M=male E. Name: animal # Description: animal identification number F. Name: lm cntl Description: littermate mouse G. Name: WT Description: wild type mouse H. Name: R6/2 Description: R6/2 transgenic mouse I. Name: M Description: male J. Name: F Description: female K. Name: [cort] ng/ml Description: concentration of corticosterone in ng/ml L. Name: mean Description: mean M. Name: SD Description: standard deviation N. Name: N Description: number of measurements ----------------------------------------- DATA-SPECIFIC INFORMATION FOR: Fig4Data.xls ----------------------------------------- 1. Number of variables: 15 2. Number of cases/rows: 55 3. Missing data codes: N/A 4. Variable List A. Name: Dex Description: Dexamethasone B. Name: time Description: time of day on 24 hr clock C. Name: age (wk) Description: age in weeks D. Name: genotype Description: genotype of mouse E. Name: sex Description: F=female, M=male F. Name: animal # Description: animals identification number G. Name: lm cntl Description: littermate mouse H. Name: R6/2 Description: R6/2 transgenic mouse I. Name: M Description: male J. Name: F Description: female K. Name: [cort] adr Description: concentration of corticosterone in ng/ml in the adrenals L. Name: [cort] sera Description: concentration of corticosterone in ng/ml in the sera M. Name: mean Description: mean N. Name: standard deviation Description: SD O. Name: N Description: number of measurements ----------------------------------------- DATA-SPECIFIC INFORMATION FOR: Fig5Data.xls ----------------------------------------- 1. Number of variables: 5 2. Number of cases/rows: 36 3. Missing data codes: N/A 4. Variable List A. Name: LM Description: littermate mouse B. Name: R6/2 Description: R6/2 transgenic mouse C. Name: M Description: Male D. Name: F Description: Female E. Name: [cort] ng/ml Description: concentration of corticosterone in ng/ml ----------------------------------------- DATA-SPECIFIC INFORMATION FOR: Fig7Data.xls ----------------------------------------- 1. Number of variables: 6 2. Number of cases/rows: 31 3. Missing data codes: N/A 4. Variable List A. Name: animal # Description: animal identification number B. Name: genotype Description: genotype of mouse C. Name: hippo Description: hippocampus D. Name: PFC Description: prefrontal cortex E. Name: PVN Description: paraventricular nucleus F. Name: STR Description: striatum