This codebook.txt file was generated on 2018/03/14 by Pranav Agrawal ------------------- GENERAL INFORMATION ------------------- 1. Title of Dataset Entropic trap purification of long DNA 2. Author Information Principal Investigator Contact Information Name: Kevin D Dorfman Institution: Department of Chemical Engineering and Material Sciences, University of Minnesota Address: 421 Washington Ave SE, Minneapolis-55414 Email: dorfman@umn.edu Associate or Co-investigator Contact Information Name: Pranav Agrawal Institution: Department of Chemical Engineering and Material Sciences, University of Minnesota Address: 421 Washington Ave SE, Minneapolis-55414 Email: agraw085@umn.edu Associate or Co-investigator Contact Information Name: Zsófia Bognár Institution: Budapest University of Technology and Economics, Budapest, Hungary Address: Email: 3. Date of data collection: 2017/01/01 - 2017/11/29 4. Geographic location of data collection: University of Minnesota 5. Information about funding sources that supported the collection of the data: NIH (R01-HG006851) -------------------------- SHARING/ACCESS INFORMATION -------------------------- 1. Licenses/restrictions placed on the data: CC0 1.0 Universal 2. Links to publications that cite or use the data: 10.1039/C7LC01355H 3. Links to other publicly accessible locations of the data: N/A 4. Links/relationships to ancillary data sets: N/A 5. Was data derived from another source? N/A 6. Recommended citation for the data: Agrawal, Pranav; Bognár, Zsófia; Dorfman, Kevin D. (2018). Entropic trap purification of long DNA. Retrieved from the Data Repository for the University of Minnesota, http://hdl.handle.net/11299/194671. -------------------------- METHODOLOGICAL INFORMATION -------------------------- 1. Description of methods used for collection/generation of data: 2. Methods for processing the data: 3. Instrument- or software-specific information needed to interpret the data: COMSOL Multiphysics software AUTOCAD Matlab 4. Standards and calibration information, if appropriate: 5. Environmental/experimental conditions: 6. Describe any quality-assurance procedures performed on the data: 7. People involved with sample collection, processing, analysis and/or submission: --------------------- DATA & FILE OVERVIEW --------------------- A. Filename: resistor_network_forFig 2.pdf Short description: Resistor model was used to build figure 2 B. Filename: Fig 2.csv Short description: The final values of potential at each nodes C. Filename: SEM Image for Figure 4.jpg Short description: Raw SEM image used in Figure 4 D. Filename: SEM Image for Figure 4-metadata.txt Short description: Metadata for SEM image used in Figure 4 E. Filename: "Fig 5a.csv" and "Fig 5b.csv" Short description: The values used to plot figure 5a and 5b. Includes time and % fluorescence intensity at 10V, 12V, or 14V. F. Filename: "Fig 6ai.csv", "Fig 6aii.csv" and "Fig 6b.csv" Short description: The values used to plot figure 6 G. Filename: "Mask Design.dxf" Short description: AUTOCAD file used to fabricate the mask for the device fabrication H. Filename: "COMSOL Model_FigSI1.mph" Short description: The 3D COMSOL model build to validate the resistor model in File A (resistor_network_forFig 2.pdf ). I. Filename: "resistor_network_COMSOL_FIgSI1.m" Short description: The Matlab code to compare the values with COMSOL model in File H (COMSOL Model_FigSI1.mph). ----------------------------------------- DATA-SPECIFIC INFORMATION FOR: Fig 2.csv ----------------------------------------- 1. Number of variables: 7 2. Number of cases/rows: 16 3. Variable List A. Name: Nodes For column A, there are 14 nodes which include the tow end nodes.There are corresponding values in column C & D. As we analyzed the data in Column E, F & G, there are 12 nodes corresponding to the 12 inner nodes (excluding the end nodes). These data were obtained using the Matlab model. B. Name: Top Channel Description: This corresponds to the electric potential (V) at each node on the device. C. Name: Bottom Channel Description: This corresponds to the electric potential (V) at each node on the device. D. Name: Electric Field Description: Electric field was calculated at each nodes but taking the difference in the voltages values in the top channel and bottom channel and divided by the length of the slit (0.96cm). E. Name: Relative Potential Drop Description: This was calculated by scaling the values with the external potential difference (12V)applied at the top and bottom channel. F. Name: Relative potential at each slit Description: This was calculated by scaling the values with the external potential difference (12V)applied at the top and bottom channel. ----------------------------------------- DATA-SPECIFIC INFORMATION FOR: Fig 5a.csv ----------------------------------------- 1. Number of variables: 2 2. Number of cases/rows: 169 3. Variable List A. Name: Time Description: Duration in seconds. This is the filtration time for each cycle. Fluorescence intensity was obtained at each time point. B. Name: % Fluorescence Intensity Description: %Fluorescent intensity was calculated for for each time point scaling the data with the maximum fluorescence intensity in each cycle. ----------------------------------------- DATA-SPECIFIC INFORMATION FOR: Fig 5b.csv ----------------------------------------- 1. Number of variables: 2 2. Number of cases/rows: 169 3. Variable List A. Name: Time Description: Duration in seconds. This is the filtration time for each cycle. Fluorescence intensity was obtained at each time point. B. Name: % Fluorescence Intensity Description: %Fluorescent intensity was calculated for for each time point scaling the data with the maximum fluorescence intensity in each cycle. ----------------------------------------- DATA-SPECIFIC INFORMATION FOR: Fig 6ai.csv ----------------------------------------- 1. Number of variables: 2 2. Number of cases/rows: 169 3. Variable List A. Name: Time Description: Duration in seconds. This is the filtration time for each cycle. Fluorescence intensity was obtained at each time point. B. Name: % Fluorescence Intensity Description: %Fluorescent intensity was calculated for for each time point scaling the data with the maximum fluorescence intensity in each cycle. ----------------------------------------- DATA-SPECIFIC INFORMATION FOR: Fig 6aii.csv ----------------------------------------- 1. Number of variables: 2 2. Number of cases/rows: 169 3. Variable List A. Name: Time Description: Duration in seconds. This is the filtration time for each cycle. Fluorescence intensity was obtained at each time point. B. Name: % Fluorescence Intensity Description: %Fluorescent intensity was calculated for for each time point scaling the data with the maximum fluorescence intensity in each cycle. ----------------------------------------- DATA-SPECIFIC INFORMATION FOR: Fig 6b.csv ----------------------------------------- 1. Number of variables: 2 2. Number of cases/rows: 132 4. Variable List A. Name: Filtration Time Description: Duration in seconds. It is the time for which filtration was performed. B. Name: Selectivity Description: it is the ratio of % Fluorescence intensity measured for lambda and 2kb DNA at the end of same filtration duration. This was calculated for various filtration time.