This readme.txt file was generated on 20250129 by Robert S. Jones Recommended citation for the data: [manuscript in submission IJMS] ------------------- GENERAL INFORMATION ------------------- 1. Title of Dataset An Oxymetazoline-based Nasal Solution Removes Bacteria-Blood Debris on Dental Surfaces and Has Antimicrobial Activity toward Streptococcus mutans Data Sharing Archive 2. Author Information Principal Investigator Contact Information Name: Robert S Jones Institution: University of Minnesota Address: 515 Delaware Street S.E.,Minneapolis, MN 55455 Email: rsjones@umn.edu ORCID: 0000-0002-9213-3657 Associate or Co-investigator Contact Information Name: Dhiraj Kumar Institution: University of Minnesota Address: 515 Delaware Street S.E.,Minneapolis, MN 55455 Email: kumard@umn.edu ORCID: 0000-0002-1008-8638 Associate or Co-investigator Contact Information Name: Morgan Pride Institution: North Carolina Agricultural and Technical State University [2023 Summer Research at University of Minnesota] Address: Greensboro, NC, 27411, USA. Email: decline to state; undergraduate student ORCID: N/A 3. Date published or finalized for release: 2025-01-29 4. Date of data collection (single date, range, approximate date) : 2023-07-10 to 2024-02-09 5. Geographic location of data collection (where was data collected?): Minneapolis, MN, USA 6. Information about funding sources that supported the collection of the data: Student supported on was partially funded by R25 DE032529 training grant (MP funding PI K Mansky). 7. Overview of the data (abstract): Data used in the manuscript An Oxymetazoline-based Nasal Solution Removes Bacteria-Blood Debris on Dental Surfaces and Has Antimicrobial Activity toward Streptococcus mutans Background: An over-the-counter vasoconstrictive nasal solution with oxymetazoline (NS-OXY, 0.05%) has potential to be used as a dental pulpal hemostatic medicament. A molecular engineering approach examined NS-OXY and its molecular constituent’s an-timicrobial and blood biomass removal efficacy. Methods: An ex vivo cavity model was developed where standardized prepared teeth were exposed overnight to a model dentinal caries pathogen, S. mutans, and then exposed to Sheep’s blood for 10 minutes, which sim-ulated a pulpal exposure. Cavity preparations were rinsed with OXY (0.05%), ben-zalkonium chloride (BKC-0.025%), NS-OXY (with OXY-0.05% and BKC), ferric sulfate (20%;ViscoStat, FS), and distilled water (DI). For examining the bactericidal effect of NS-OXY, a disc diffusion antimicrobial assay was used where S. mutans was grown (20-hrs) on brain-heart infusion (BHI) w/0.5% glucose agar plates and exposed to the treatment groups. Results: NS-OXY treated samples had a lower residual bacterial or blood biomass than FS (P=.003). The diffusion test showed that NS-OXY, BKC, and FS had zones of inhibition greater than 10 mm, with NS-OXY having higher activity against S. mutans than FS (P=.0002) but lower than BKC (P=0.0082). Conclusions: NS-OXY may con-sidered as a dental hemostatic agent after traumatic and carious pulpal exposure owing to NS-OXY’s antimicrobial and vasoconstrictive properties. -------------------------- SHARING/ACCESS INFORMATION -------------------------- 1. Licenses/restrictions placed on the data: CC0 1.0 Universal 2. Links to publications that cite or use the data: [manuscript in submission IJMS] 3. Was data derived from another source?No If yes, list source(s): 4. Terms of Use: Data Repository for the U of Minnesota (DRUM) By using these files, users agree to the Terms of Use. https://conservancy.umn.edu/pages/drum/policies/#terms-of-use --------------------- DATA & FILE OVERVIEW --------------------- 1. File List A. Filename: Figure_2_DRUM_Jones_Biomass.csv Short description: Main Text and Supplemental info Data that supports publication B. Filename: Figure_3_DRUM_Jones_Disc_inhibition.csv Short description: Main Text and Supplemental info Data that supports publication C. Filename: Figure_4_DRUM_Jones_pH.csv Short description: Main Text and Supplemental info Data that supports publication D. Filename: Figure_4_DRUM_Jones_OD.csv Short description: Main Text and Supplemental info Data that supports publication 2. Relationship between files: Main text and supplemental data -------------------------- METHODOLOGICAL INFORMATION -------------------------- 1. Description of methods used for collection/generation of data: a) Ex vivo bacteria and pulpal exposure model used a cavity preparation in dentin followed by overnight incubation in presence of S. mutans in BHI media and subsequent exposure to Sheep’s blood (5 min). Bacteria-blood was exposed to treatment (10 sec) and rinsed with 2 ml of dis-tilled water. Residual debris was stained and quantified with crystal violet and spectroscopic analysis. Treatment groups were a) control-distilled water b) NS-OXY that contained 0.05% OXY c) 0.025% BKC d) 0.05 % OXY and e) 20% ferric sulfate (FS). b) For examining the bactericidal effect of NS-OXY, a disc diffusion antimicrobial assay was used where S. mutans was grown (20-hrs) on brain-heart infusion (BHI) w/0.5% glucose agar plates and exposed to the treatment groups. c) BHI broth cultures of Streptococcus mutans were exposed to three of the above treatment groups (n=3) in addition to a non-treatment control. Aliquots were sampled periodically during 24 hrs to examine growth (OD) and media pH. 2. Methods for processing the data: To test for differences between treatment groups (NS-OXY, OXY, BKC, FS, and sterile dis-tilled water (control)) on S. mutans growth and residual biomass, a 1-way ANOVA was performed 3. Instrument- or software-specific information needed to interpret the data: Data presented in .csv format 4. Standards and calibration information, if appropriate: see publication link: controls are listed in data set. 5. Environmental/experimental conditions: [see manuscript with same title] 6. Describe any quality-assurance procedures performed on the data: [see manuscript with same title] 7. People involved with sample collection, processing, analysis and/or submission: sample collection, processing: M Pride, D Kumar, RS Jones