This zip file contains all 16S raw paired end reads sequencing data that we used to characterize the Ixodes scapularis microbiome community across tick populations in the upper midwest.

Samples are coded as follows (Site Code)(Year of sample collection)_#(Tick sex or Sample type) followed by non-necessary inforamation on plate well and barcoding
Note that each sample will have two sequences denoted by the R1 or R2 at the trailing end before 001. As Illumina miseq generates paired end reads.

List of Site Codes and coordinates to the location of sampling sites are listed below
Site Code	Coordinates
CNF	47.49541, -93.65031
IS	47.19567, -95.2576
CR	46.10728, -94.37419
CA	45.35073, -93.0312
KF	45.31014, -93.03169
SC	45.95399, -92.73269
WW	44.056946, -92.049376
RDWB	43.73793, -91.64205
YR	43.178278, -91.240302
C, CE	42.67699, -91.59584
T, B	45.94043, -91.80924
TSA	45.21141, -91.38059
TH, SG	43.16581, -90.04027
GMWM, GMW	45.37001, -93.11268
LC	47.04312, -91.70883
HI	47.9982, -96.28737

Years are all in 2000. Thus for samples coded with 17 for the year of sample collection, ticks were collected from that site in the summer of 2017

Tick Sex is either M for male or F for Female. S coded samples are soil samples

Samples that do not follow the site code and year breakdown are either labeled BLANK or BRACHYSPIRA. Blank samples are blank wells used as negative controls and BRACHYSPIRA samples are splash control wells.