This codebook.txt file was generated on <20210211> by ------------------- GENERAL INFORMATION ------------------- 1. Title of Dataset: Data for S-layer glycosylation supports surface-associated growth and iron oxidation in Methanococcus maripaludis 2. Author Information Contact Information Name: Kyle C. Costa Institution: University of Minnesota Email: kcosta@umn.edu ORCID: 0000-0003-0407-1431 Contact Information Name: Matthew P. Holten Institution: University of Minnesota Email: ORCID: Contact Information Name: Dallas R. Fonseca Institution: University of Minnesota Email: fonse039@umn.edu ORCID: 3. Date of data collection (single date, range, approximate date) 2020/07/30 - 2020/08/25 4. Geographic location of data collection (where was data collected?): University of Minnesota Center for Mass Spectrometry and Proteomics 5. Information about funding sources that supported the collection of the data: Sponsorship: Young Investigator Program award to KCC from the Army Research Office, grant number W911NF-19-1-0024; DRF was supported by the National Science Foundation Graduate Research Fellowship Program under grant number CON-75851. -------------------------- SHARING/ACCESS INFORMATION -------------------------- 1. Licenses/restrictions placed on the data: CC0 1.0 Universal 2. Links to publications that cite or use the data: 3. Links to other publicly accessible locations of the data: 4. Links/relationships to ancillary data sets: 5. Was data derived from another source? If yes, list source(s): 6. Recommended citation for the data: Holten, Matthew P; Fonseca, Dallas R; Costa, Kyle C. (2021). Data for S-layer glycosylation supports surface-associated growth and iron oxidation in Methanococcus maripaludis. Retrieved from the Data Repository for the University of Minnesota, https://doi.org/10.13020/fhxh-n267. --------------------- DATA & FILE OVERVIEW --------------------- 1. File List A. Filename: kcosta_fonse039_20200819_17778_DF_1_3ul Short description: Mass Spectrometry data of a single band cut from a polyacrylamide gel. Genotype of strain is Methanococcus maripaludis type strain S2 WT. B. Filename: kcosta_fonse039_20200819_17778_DF_2_4ul Short description: Mass Spectrometry data of a single band cut from a polyacrylamide gel. Genotype of strain is Methanococcus maripaludis type strain JJ WT. This sample is of the top band on the gel. C. Filename: kcosta_fonse039_20200819_17778_DF_3_4ul Short description: Mass Spectrometry data of a single band cut from a polyacrylamide gel. Genotype of strain is Methanococcus maripaludis type strain JJ WT. This sample is of the bottom band on the gel. D. Filename: kcosta_fonse039_20200819_17778_DF_4 Short description: Mass Spectrometry data of a single band cut from a polyacrylamide gel. Genotype of strain is Methanococcus maripaludis type strain JJ ΔaglB. 2. Relationship between files: Each file is a different band cut from a polyacrylamide gel then submitted for protein identity to the Cetner for Mass Spectrometry and Proteomics. 3. Software for opening files: Images may be opened using open source software ImageJ. METHODOLOGICAL INFORMATION -------------------------- 1. Description of methods used for collection/generation of data: To purify the S-layer glycoprotein, strains were grown overnight in McCas medium to a final OD600 of 0.8 to 1.0. Tubes we opened under an oxic atmosphere, and 4.5 ml of culture material was collected by centrifugation in 1.7 ml microcentrifuge tubes (3 spins at 13,000 xg for 1 minute). Residual supernatant was removed and the cell pellet was suspended in 0.6 ml of protoplasting buffer (0.35 M sucrose, 0.38 M NaCl, 50 mM Tris, pH 7.5) by gentle pipetting (35). Cells were pelleted by centrifugation (13,000 xg for 1 minute), and the supernatant was concentrated to ~50 µl using a Vivaspin 500 column (3 kDa molecular weight cutoff) protein concentrator following manufacturer’s instructions. The concentrated supernatant was subjected to sodium dodecyl sulfate polyacrylamide gel electrophoresis (SDS-PAGE) using a 4–20% Criterion™ TGX™ Precast Midi protein gel (BioRad) followed by staining with Coomassie Brilliant Blue R-250. A second gel was used to cut out bands from proteins purified from wild-type strain S2, wild-type strain JJ, and the strain JJ ∆aglB mutant strain. These cut bands were submitted to the University of Minnesota Center for Mass Spectrometry and Proteomics. 2. Methods for processing the data: samples (log number 17778) were analyzed on the Orbitrap Velos mass spectrometer 3. Instrument- or software-specific information needed to interpret the data: PEAKS X Studio software was used for data analysis 4. Standards and calibration information, if appropriate: 5. Environmental/experimental conditions: 6. Describe any quality-assurance procedures performed on the data: 7. People involved with sample collection, processing, analysis and/or submission: Dallas Fonseca collected and submitted the samples to the Center for Mass Spectrometry and Proteomics (CMSP). The CMSP then processed the data. Dallas Fonseca then reviewed/analyzed the data in Peaks X.