This readme.txt file was generated on 20240712 by Aset Khakimzhan
Recommended citation for the data: Cell-free expression with a quartz crystal microbalance enables rapid, dynamic, and label-free characterization of membrane-interacting proteins


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GENERAL INFORMATION
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1. Cell-free expression with a quartz crystal microbalance enables rapid, dynamic, and label-free characterization of membrane-interacting proteins


2. Author Information


  Principal Investigator Contact Information
        Name: Vincent Noireaux
           Institution: University of Minnesota - Twin Cities
           Address: 115 Union Street SE, 55455, Minneapolis, MN
           Email: noireaux@umn.edu
	   ORCID: 0000-0002-5213-273X

  Associate or Co-investigator Contact Information
        Name: Aset Khakimzhan
           Institution: University of Minnesota - Twin Cities
           Address: 115 Union Street SE, 55455, Minneapolis, MN
           Email: khaki005@umn.edu
	   ORCID: 0000-0002-2258-9085

  Associate or Co-investigator Contact Information
        Name: Ziane Izri
           Institution: University of Minnesota - Twin Cities
           Address: 115 Union Street SE, 55455, Minneapolis, MN
           Email: izri0001@umn.edu
	   ORCID: 0000-0003-4074-1535

  Associate or Co-investigator Contact Information
        Name: Seth Thompson
           Institution: University of Minnesota - Twin Cities
           Address: 115 Union Street SE, 55455, Minneapolis, MN
           Email: thom5884@umn.edu
	   ORCID: 0000-0003-1713-223X

  Associate or Co-investigator Contact Information
        Name: Oleg Dmytrenko
           Institution: Helmholtz Institute for RNA-based Infection Research (HIRI), Helmholtz-Centre for Infection Research (HZI), 
           Address: Josef-Schneider-Straße 2/D15, 97080 Würzburg, Germany
           Email: oleg.dmytrenko@helmholtz-hiri.de
	   ORCID: 0000-0002-1165-6836

  Associate or Co-investigator Contact Information
        Name: Patrick Fischer
           Institution: Helmholtz Institute for RNA-based Infection Research (HIRI), Helmholtz-Centre for Infection Research (HZI), 
           Address: Josef-Schneider-Straße 2/D15, 97080 Würzburg, Germany
           Email: patrick.fischer@stud-mail.uni-wuerzburg.de
	   ORCID: n/a

 Associate or Co-investigator Contact Information
        Name: Chase Beisel
           Institution: Helmholtz Institute for RNA-based Infection Research (HIRI), Helmholtz-Centre for Infection Research (HZI), 
           Address: Josef-Schneider-Straße 2/D15, 97080 Würzburg, Germany
           Email: chase.beisel@helmholtz-hiri.de
	   ORCID: 0000-0003-0650-9943


3. Date published or finalized for release: 20240115

4. Date of data collection (single date, range, approximate date)
	Start: 20221101
	End: 20240505

5. Geographic location of data collection (where was data collected?):  
	Minneapolis, USA: TXTL data
	Würzburg, Germany: Zorya infection data

6. Information about funding sources that supported the collection of the data: 
	NSF Award Abstract # 2017932
	BBSRC-NSF/BIO: Real-time Nanosensing of Membrane Protein Assembly in a TXTL Synthetic Cell System

7. Overview of the data (abstract): 
	Quartz crystal microbalance raw data. The data contains the process of SLB formation (usually within the first 4 hours of data collection) and then incubation of TXTL with the SLB (rest of the measurement).
	Fluorescence raw data and analysis. Contain raw data readouts from a fluorescence plate reader. Also contain the analysis that groups conditions for averaging and standard deviation calculations. 
	Phage titration data. Contains PFU information for multiple conditions. Also contains the analysis that groups conditions for averages and standard deviation calculations. 

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SHARING/ACCESS INFORMATION
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1. Licenses/restrictions placed on the data: N/A

2. Links to publications that cite or use the data: https://www.biorxiv.org/content/10.1101/2024.01.14.575553v1

3. Was data derived from another source? No

4. Terms of Use: Data Repository for the U of Minnesota (DRUM) By using these files, users agree to the Terms of Use. https://conservancy.umn.edu/pages/policies/#drum-terms-of-use




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DATA & FILE OVERVIEW
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1. File List - 'QCMD figure data map.csv' additional excel file contains the entire file list and description of conditions.

2. Relationship between files: No relationship between any of the files. 



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METHODOLOGICAL INFORMATION
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1. Description of methods used for collection/generation of data: QCMD-TXTL, Fluorescence measurements, Phage titration assays

2. Methods for processing the data: No data processing was performed.


3. Instrument- or software-specific information needed to interpret the data: N/A


4. Standards and calibration information, if appropriate: N/A


5. Environmental/experimental conditions: TXTL: 29C, 1 atm. Infection assays: 37C


6. Describe any quality-assurance procedures performed on the data: N/A


7. People involved with sample collection, processing, analysis and/or submission: Aset Khakimzhan, Ziane Izri, Seth Thompson, 
	Oleg Dmytrenko, Patrick Fischer, Chase Beisel, Vincent Noireaux



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DATA-SPECIFIC INFORMATION FOR: [FILENAME]
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<create sections for each dataset included>


1. Number of variables:


2. Number of cases/rows: 




3. Missing data codes:
        Code/symbol        Definition
        Code/symbol        Definition


4. Variable List
                  

    A. Name: <variable name>
       Description: <description of the variable>
                    Value labels if appropriate


    B. Name: <variable name>
       Description: <description of the variable>
                    Value labels if appropriate