This readme.txt file was generated on <2020-01-11> by <Vincent Noireaux>


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GENERAL INFORMATION
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1. Title of Dataset: Data from: Membrane molecular crowding enhances MreB polymerization to shape synthetic cells from sphere to rods.


2. Author Information


  Principal Investigator Contact Information
        Name: Vincent Noireaux
           Institution: University of Minnesota
           Address: 115 Union Street SE, Minneapolis MN 55455
           Email: noireaux@umn.edu
	   ORCID: https://orcid.org/0000-0002-5213-273X 

  Associate or Co-investigator Contact Information
        Name: David Garenne
           Institution: University of Minnesota
           Address: 115 Union Street SE, Minneapolis MN 55455
           Email: david.garenne@hotmail.fr
	   ORCID: NA

  Associate or Co-investigator Contact Information
        Name: Albert Libchaber
           Institution: The Rockefeller University
           Address: 1230 York Avenue, New York, NY 10021, USA
           Email: libchbr@mail.rockefeller.edu
	   ORCID: NA


3. Date of data collection (single date, range, approximate date): 

2019-01-01 to 2019-11-31


4. Geographic location of data collection (where was data collected?): at the University of Minnesota, Minneapolis campus, Physics and Nanotechnology building.


5. Information about funding sources that supported the collection of the data: This work is supported by the Human Frontier Science Program grant number RGP0037/2015.


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SHARING/ACCESS INFORMATION
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1. Licenses/restrictions placed on the data:

CC0 1.0 Universal

2. Links to publications that cite or use the data:

D. Garenne, A. Libchaber, V. Noireaux, Membrane molecular crowding enhances MreB polymerization to shape synthetic cells from sphere to rods. PNAS. https://doi.org/10.1073/pnas.1914656117

3. Links to other publicly accessible locations of the data:


4. Links/relationships to ancillary data sets:


5. Was data derived from another source?
           If yes, list source(s):


6. Recommended citation for the data: 

Noireaux, Vincent; Garenne, David. (2020). Data from: Membrane molecular crowding enhances MreB polymerization to shape synthetic cells from sphere to rods. Retrieved from the Data Repository for the University of Minnesota, http://hdl.handle.net/11299/210183. 

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DATA & FILE OVERVIEW
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1. File List

Figure 2.xlsx	
Figure 3.xlsx	
Figure 5.xlsx	
Figure S2.xlsx	
Figure S3.xlsx
Figure S4.xlsx	
Figure S5.xlsx
Figure S6.xlsx
Figure S7.xlsx
Figure S8.xlsx
Figure S9.xlsx
Figure S12.xlsx
Figure S13.xlsx	
Figure S14.xlsx

Detailed information about each figure can be found in the SI of the associated manuscript: https://doi.org/10.1073/pnas.1914656117

2. Relationship between files:        

Files contain the data used in the accompanying article, and are named by the figure numbers

3. Additional related data collected that was not included in the current data package:


4. Are there multiple versions of the dataset? 

No
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METHODOLOGICAL INFORMATION
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1. Description of methods used for collection/generation of data: 
Most of the data were collected by fluorescence microscopy (classic one photon inverted microscope) and by fluorometry (plate reader equipped with a monochromator).
Useful references: 
• liposome protocol: Garamella J, Garenne D, Noireaux V (2019) TXTL-based approach to synthetic cells. Methods Enzymol. doi:10.1016/bs.mie.2018.12.015.
• the experimental platform use in this work is an E. Coli cell-free system described in: Garamella J, Marshall R, Rustad M, Noireaux V (2016) The All E. coli TX-TL Toolbox 2.0: A Platform for Cell-Free Synthetic Biology. ACS Synth Biol. doi:10.1021/acssynbio.5b00296.


2. Methods for processing the data:
Fluorescence images were analyzed using the software Metamorph. The long and short axis for each liposome were measured to plot histograms. The fluorescence data from the plate readers (batch mode cell-free reactions) were converted into eGFP concentration using a quantitative calibration.


3. Instrument- or software-specific information needed to interpret the data:
Microscopy image: Metamorph
Plate reader data: MS Excel, Kaleidagraph


4. Standards and calibration information, if appropriate:
Calibration of fluorescence microscopy: using pure quantified eGFP protein commercially available
Calibration of the plate readers: using pure quantified eGFP protein commercially available (linear regime)


5. Environmental/experimental conditions: standard laboratory conditions


6. Describe any quality-assurance procedures performed on the data: the experiments were replicated 3 times for reproducibility. 


7. People involved with sample collection, processing, analysis and/or submission: David Garenne and Vincent Noireaux