This readme.txt file was generated on 2021/09/19 by Natalie McMann, Alexander Peichel, and Jessica Savage

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GENERAL INFORMATION
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1. Title of Dataset 

Floral gas exchange data observed from cut and intact inflorescences and during a heating experiment in 2018 on woody plants growing in Duluth, MN

2. Author Information

  Principal Investigator Contact Information
        Name: Jessica A. Savage	
           Institution: University of Minnesota
           Address: 1035 Kirby Drive, 207 Swenson Science Building, Duluth, MN 55812
           Email: jsavage@d.umn.edu
	   ORCID: 0000-0002-7756-7166

  First Author Contact Information
	Name: Natalie McMann
	   Institution: University of Minnesota
           Address: 1035 Kirby Drive, 207 Swenson Science Building, Duluth, MN 55812
           Email: mcman110@d.umn.edu
	   ORCID: 0000-0001-5275-7775 

  Second Author Contact Information
	Name: Alexander Peichel
	Institution: University of Minnesota
	Address: 1035 Kirby Drive, 207 Swenson Science Building, Duluth, MN 55812
	Email: 
	ORCID: NA

3. Date of data collection (single date, range, approximate date) <suggested format YYYYMMDD>

2018/05/10-2018/06/05

4. Geographic location of data collection (where was data collected?): 

Bagley Nature Area, Duluth, MN —(46.8241° N, 92.0868° W)
University of Minnesota Duluth Campus, Duluth, MN —(46° 49' 10.25" N, 92° 04' 54.12" W)
Central Hillside, Duluth, MN —(46°47'44.8”N, 92°06'17.6"W)

5. Information about funding sources that supported the collection of the data:

National Science Foundation (IOS:1656318), the University of Minnesota – Duluth, the Garden Club of America (Corliss Knapp Engle Scholarship) and the Integrated Biosciences Graduate Program

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SHARING/ACCESS INFORMATION
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1. Licenses/restrictions placed on the data:

Manuscript copyright held by Wiley Online Library

2. Links to publications that cite or use the data:

McMann, N., Peichel A., & Savage, J. 2022. Early spring flowers rely on xylem hydration but are not limited by stem xylem conductivity. New Phytologist, 233(2), 838-850.  

3. Links to other publicly accessible locations of the data:

None

4. Links/relationships to ancillary data sets:

None

5. Was data derived from another source?
           If yes, list source(s):

No

6. Recommended citation for the data:

McMann, N., Peichel A., & Savage, J. 2022. Early spring flowers rely on xylem hydration but are not limited by stem xylem conductivity. New Phytologist, 233(2), 838-850.  

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DATA & FILE OVERVIEW
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1. File List
   A. Filename: Floral_cutting 
      Short description: This file contains data on the diffusive conductance, transpiration, and respiration of inflorescences during anthesis.

   B. Filename: Floral_heating    
      Short description: This file contains data on the diffusive conductance of mature leaves during the growing season.

2. Relationship between files: All of these files are data collected during a two year project investigating the relationship between flowering and plant vasculature.     

3. Additional related data collected that was not included in the current data package:

Yes, there are two additional data packages: 

McMann, N., Peichel A., and Savage, J. (2021). Floral and leaf gas exchange and stem hydraulic conductance data observed from 2017 to 2018 on woody plants growing in Minnesota
McMann, N., Peichel A., and Savage, J. (2021). Plant phenology and floral anatomy data observed from 2017 to 2020 on woody plants growing in Minnesota

4. Are there multiple versions of the dataset? yes/no

No

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METHODOLOGICAL INFORMATION
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1. Description of methods used for collection/generation of data: 

See descriptions under “Variable Lists” below and publication for details. 

	A. Floral_cutting
	Inflorescence gas exchange data was collected using a portable gas exchange analyzer (6400, LI-COR, Lincoln, NE) for Salix discolor and Rhododendron x in spring 2018. Measurements were taken during anthesis on 16-20 inflorescences per species using a clear LI-COR conifer chamber (6400-05). The day before taking gas exchange measurements, we selected 6-7 branches from an individual plant of each species and excised them underwater in the evening. These cut branches were placed in containers of water next to the plant and left for 12 hours to adjust to ambient conditions. Gas exchange measurements on 8-10 cut and 8-10 intact inflorescences took place the next morning under ambient field conditions with a flow rate of 500 µmol s-1 using a 20-L carboy as an air buffer. 

	B. Floral_heating
	Inflorescence gas exchange data was collected using a portable gas exchange analyzer (6400, LI-COR, Lincoln, NE) during a heating experiment on Salix discolor, Prunus americana, and Rhododendron x  inflorescences in spring 2018. Measurements were taken during anthesis on seven inflorescences per species using a clear LI-COR conifer chamber (6400-05) at four temperatures: ambient (~20–23°C), 26°C, 28°C, and 30°C. We heated the chamber with an infrared light (125R40/HT, No. 0390700, Westinghouse Lighting, Philadelphia, PA) and monitored temperature with the LI-COR chamber thermocouple and an independent thermocouple (TC6-T, HOBO UX100-014M, Onset Computer Corporation, Bourne, MA) touching the inflorescence. Measurements occurred in the laboratory under a grow light (SunBlaze T5HO-44, Sunlight Supply, Inc., Vancouver, WA) with a PAR range of 180-240 µmol m-2 s-1, at a flow rate of 500 µmol s-1 using a 20-L carboy as an air buffer, and ambient CO2 concentrations of 420-430 ppm and relative humidity between 20-31%.

2. Methods for processing the data: 
NA

3. Instrument- or software-specific information needed to interpret the data:

NA

4. Standards and calibration information, if appropriate:

NA

5. Environmental/experimental conditions:

NA

6. Describe any quality-assurance procedures performed on the data:

All data entry was triple checked.

7. People involved with sample collection, processing, analysis and/or submission:

Natalie McMann, Jessica Savage, Alexander Peichel, Erin O’Connell, Kennedy Mosher, and Thomas Kiecker

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DATA-SPECIFIC INFORMATION FOR: Floral_cutting
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1. Number of variables: 10

2. Number of cases/rows: 29

3. Missing data codes:
     NA

4. Variable List

    A. Name: species
       Description: Scientific name of the species

    B. Name: treatment
       Description: This is the experimental treatment that an inflorescence received prior to gas exchange measurements. The treatments are coded as “cut”, meaning that the branch bearing the inflorescence was cut from the plant, or “intact”, meaning that the inflorescence is from a branch that remained on the plant. 

    C. Name: rep
       Description: This is the sample number of the inflorescence. 
 
    D. Name: cond
       Description: This is the diffusive conductance of the inflorescence standardized by the surface area of inflorescence in the LI-COR chamber (mol m-2 s-1). 

    E. Name: trmmol
       Description: This is the transpiration of the inflorescence standardized by the surface area of inflorescence in the LI-COR chamber (mmol m-2 s-1). 

    F. Name: vpd
       Description: This is the vapor pressure deficit (kPa) in the LI-COR chamber during measurement. 

    G. Name: par
       Description: This is the light level in the chamber (µmol m^-2 s^-1).

    H. Name: rh
       Description: This is the relative humidity (%) of the reference air during measurement.

    I. Name: temp
       Description: This is the temperature of the inflorescence in the LI-COR chamber (°C).

    J. Name: collection date
	  Description: This is the date that inflorescences were collected and measured. 

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DATA-SPECIFIC INFORMATION FOR: Floral_heating
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1. Number of variables: 11

2. Number of cases/rows: 85

3. Missing data codes:
     NA

4. Variable List

    A. Name: species
       Description: Scientific name of the species

    B. Name: rep
       Description: This is the sample number of the inflorescence. 

    C. Name: treatment
       Description: This is the experimental treatment that an inflorescence received prior to gas exchange measurements, coded as “ambient”, meaning the inflorescence and LI-COR chamber were not heated prior to measurement, “heat26”, meaning the inflorescence and chamber were heated to 26°C, “heat28”, meaning the inflorescence and chamber were heated to 28°C, “heat30”, meaning the inflorescence and chamber were heated to 30°C.
 
    D. Name: temp
       Description: This is the inflorescence temperature (°C) recorded during measurement. 

    E. Name: rh
       Description: This is the relative humidity (%) of the reference air during measurement. 

    F. Name: vpd
       Description: This is the vapor pressure deficit (kPa) in the LI-COR chamber during measurement. 

    G. Name: par
       Description: This is the light level (µmol m^-2 s^-1) in the chamber during measurement.

    H. Name: co2
       Description: This is CO2 concenteration (ppm) in the reference air during measurement.

    I. Name: trmmol
       Description: This is the transpiration of the inflorescence standardized by the surface area of inflorescence in the chamber (mmol m-2 s-1). 

    J. Name: cond
       Description: This is the diffusive conductance of the inflorescence standardized by the surface area of inflorescence in the LI-COR chamber (mol m-2 s-1). 

    K. Name: collection date
	  Description: This is the date that inflorescences were collected and measured.