The research is relevant to 18 million Americans, primarily the elderly, who suffer
from osteopenia (loss of bone mass) and osteoporosis. The other targeted
population is astronauts, in low-earth orbit, who lose 1 to 3 % of their bone mass
per month. 1H-1DNMR (700MHz) spectra of water soluble extracts from MC3T3
Osteoblastic cells were obtained. Two osteoblast populations were studied – one
cultured in cell media with the addition of ascorbic acid and the other population
cultured in cell media without the addition of ascorbic. Ascorbic acid induces
osteoblast cells to differentiate resulting in the production of bone. The
metabolomic signature of osteoblast cells, pre and post differentiation, were
compared through 1H-1DNMR spectroscopy . 1H NMR Spectra was analyzed by
Chenomx 6.1 software which is available at the University of Minnesota
Biomedical NMR facility. Chenomx deconvolves the chemical shifts of an NMR
spectrum and assigns them to known metabolite signatures. This allows the
identification of roughly 100 to 200 metabolites. Chemical shifts that Chenomx did
not have in its library was assigned using 1H-2D-NOSEY NMR spectroscopy. The
techniques developed here will be used in future experiments for cells that are
magnetically levitated to observe changes in cell metabolomics when gravitational
loading is reduced or eliminated.
Additional contributors: Louis S. Kidder; Bruce E. Hammer (faculty mentor).
This project continuation is supported by the Undergraduate Research Opportunities Program (UROP) and computational resources from Minnesota Supercomputing Institute for Advanced Computational Research of the University of Minnesota.
Kim, Hee Eun.
NMR Metabolomic Analysis of Osteoblasts Pre and Post Differentiation.
Retrieved from the University of Minnesota Digital Conservancy,
Content distributed via the University of Minnesota's Digital Conservancy may be subject to additional license and use restrictions applied by the depositor.